| Item type |
デフォルト(1) |
| 公開日 |
2025-07-16 |
| タイトル |
|
|
タイトル |
Sensitive and Specific MicroRNA In Situ Hybridization Using Partially Methylated Phosphotriester Antisense DNA Probes |
|
言語 |
en |
| 作成者 |
Wang, Po-Hsiang
Jia, Tony Z.
Chang, Ching-Wen
Tan, Bertrand Chin-Ming
Chi, Ya-Hui
Chen, Wen-Yih
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is the accepted version of the following article: Po-Hsiang Wang, Tony Z. Jia, Ching-Wen Chang, Bertrand Chin-Ming Tan, Ya-Hui Chi, and Wen-Yih Chen. Sensitive and Specific MicroRNA In Situ Hybridization Using Partially Methylated Phosphotriester Antisense DNA probes. GEN Biotechnology, 1(5), 447-455 (2022), which has now been formally published in final form at GEN Biotechnology at https://doi.org/10.1089/genbio.2022.0023 This original submission version of the article may be used for non-commercial purposes in accordance with the Mary Ann Liebert, Inc., publishers’ self-archiving terms and conditions. |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is not the published version. Please cite only the published version. |
| 権利情報 |
|
|
言語 |
ja |
|
権利情報 |
この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Antisense oligonucleotide probe |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
neutralized DNA |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
modified nucleic acid |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
in situ hybridization |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
microRNA |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
colorectal cancer |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Neutralized DNA (nDNA) is an emerging class of DNA oligonucleotides chemically synthesized with site-specific internucleoside methyl phosphotriester (MPTE) linkages, changing the negatively charged DNA phosphodiester backbone to a neutral MPTE backbone. nDNA was used as sensitive probes for nucleotide sequencing; however, the application of nDNA for microRNAs (miRNA) inhibition and ex vivo RNA hybridization remains largely unexplored. In this study, we determined that partially methylated nDNA probes inhibited reverse transcription of oncogenic miRNA miR-21 in synthetic human plasma more efficiently than canonical DNA/highly methylated nDNA probes and with an efficiency comparable with locked nucleic acid probes at nM concentrations. Moreover, in situ hybridization using a miR-21-expressing colorectal cancer cell HCT116 stained with partially methylated nDNA probes revealed a greater efficacy than HCT116 stained with canonical DNA probes. Given that the N4 nDNA probe is immune-negative and endonuclease-resistant, partially methylated nDNA could be further developed to have significant applications in biotechnology and medicine. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述 |
This study is supported by the Ministry of Science and Technology of Taiwan (MOST) (110-2222-E-008-002-MY2; 108-2221-E-008-056-MY3). Tony Z. Jia is supported by the Japan Society for the Promotion of Science (JSPS) (Grants-in-aid 21K14746). Po-Hsiang Wang is also supported by the Research and Development Office as well as Research Center for Sustainable Environmental Technology, National Central University, Taiwan. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Mary Ann Liebert |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1089/genbio.2022.0023 |
| 助成情報 |
|
|
|
助成機関識別子タイプ |
Crossref Funder |
|
|
助成機関識別子タイプURI |
https://doi.org/10.13039/501100001691 |
|
|
助成機関名 |
日本学術振興会 |
|
|
言語 |
ja |
|
|
助成機関名 |
Japan Society for the Promotion of Science |
|
|
言語 |
en |
|
|
研究課題番号URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-21K14746/ |
|
|
研究課題番号 |
21K14746 |
|
|
研究課題名 |
Biomolecule compartmentalization by phase separated protocells |
|
|
言語 |
en |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2768-1572 |
| 書誌情報 |
en : GEN Biotechnology
巻 1,
号 5,
p. 447-455,
発行日 2022-10-24
|
genbio_1-5_447.docx (89 KB)
