{"created":"2025-07-15T06:53:43.857404+00:00","id":2040795,"links":{},"metadata":{"_buckets":{"deposit":"67f319aa-34e6-44c8-9b95-1f8294d4ac56"},"_deposit":{"created_by":83,"id":"2040795","owner":"83","owners":[83],"pid":{"revision_id":0,"type":"depid","value":"2040795"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02040795","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Sensitive and Specific MicroRNA In Situ Hybridization Using Partially Methylated Phosphotriester Antisense DNA Probes","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Wang, Po-Hsiang","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Jia, Tony Z.","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Chang, Ching-Wen","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tan, Bertrand Chin-Ming","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Chi, Ya-Hui","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Chen, Wen-Yih","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"This is the accepted version of the following article: Po-Hsiang Wang, Tony Z. Jia, Ching-Wen Chang, Bertrand Chin-Ming Tan, Ya-Hui Chi, and Wen-Yih Chen.  Sensitive and Specific MicroRNA In Situ Hybridization Using Partially Methylated Phosphotriester Antisense DNA probes.  GEN Biotechnology, 1(5), 447-455 (2022), which has now been formally published in final form at GEN Biotechnology at https://doi.org/10.1089/genbio.2022.0023   This original submission version of the article may be used for non-commercial purposes in accordance with the Mary Ann Liebert, Inc., publishers’ self-archiving terms and conditions.","subitem_rights_language":"en"},{"subitem_rights":"This is not the published version. Please cite only the published version.","subitem_rights_language":"en"},{"subitem_rights":"この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。","subitem_rights_language":"ja"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Antisense oligonucleotide probe","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"neutralized DNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"modified nucleic acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"in situ hybridization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"colorectal cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Neutralized DNA (nDNA) is an emerging class of DNA oligonucleotides chemically synthesized with site-specific internucleoside methyl phosphotriester (MPTE) linkages, changing the negatively charged DNA phosphodiester backbone to a neutral MPTE backbone. nDNA was used as sensitive probes for nucleotide sequencing; however, the application of nDNA for microRNAs (miRNA) inhibition and ex vivo RNA hybridization remains largely unexplored. In this study, we determined that partially methylated nDNA probes inhibited reverse transcription of oncogenic miRNA miR-21 in synthetic human plasma more efficiently than canonical DNA/highly methylated nDNA probes and with an efficiency comparable with locked nucleic acid probes at nM concentrations. Moreover, in situ hybridization using a miR-21-expressing colorectal cancer cell HCT116 stained with partially methylated nDNA probes revealed a greater efficacy than HCT116 stained with canonical DNA probes. Given that the N4 nDNA probe is immune-negative and endonuclease-resistant, partially methylated nDNA could be further developed to have significant applications in biotechnology and medicine.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"This study is supported by the Ministry of Science and Technology of Taiwan (MOST) (110-2222-E-008-002-MY2; 108-2221-E-008-056-MY3). Tony Z. Jia is supported by the Japan Society for the Promotion of Science (JSPS) (Grants-in-aid 21K14746). Po-Hsiang Wang is also supported by the Research and Development Office as well as Research Center for Sustainable Environmental Technology, National Central University, Taiwan.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Mary Ann Liebert","subitem_publisher_language":"en"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186901218":{"attribute_name":"Funding Reference","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"21K14746","subitem_award_uri":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-21K14746/"},"subitem_award_titles":[{"subitem_award_title":"Biomolecule compartmentalization by phase separated protocells","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier_type":"Crossref Funder","subitem_funder_identifier_type_uri":"https://doi.org/10.13039/501100001691"},"subitem_funder_names":[{"subitem_funder_name":"日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"2768-1572","subitem_source_identifier_type":"ISSN"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-10-24","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"455","bibliographicPageStart":"447","bibliographicVolumeNumber":"1","bibliographic_titles":[{"bibliographic_title":"GEN Biotechnology","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1089/genbio.2022.0023","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-07-16"}],"filename":"genbio_1-5_447.docx","filesize":[{"value":"89 KB"}],"format":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2040795/files/genbio_1-5_447.docx"},"version_id":"66385af7-d97b-41f7-a0cc-c4f3166613a7"}]},"item_title":"Sensitive and Specific MicroRNA In Situ Hybridization Using Partially Methylated Phosphotriester Antisense DNA Probes","item_type_id":"40036","owner":"83","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-07-16"},"publish_date":"2025-07-16","publish_status":"0","recid":"2040795","relation_version_is_last":true,"title":["Sensitive and Specific MicroRNA In Situ Hybridization Using Partially Methylated Phosphotriester Antisense DNA Probes"],"weko_creator_id":"83","weko_shared_id":-1},"updated":"2025-07-15T07:32:36.316777+00:00"}