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  1. 広島大学の刊行物
  2. Hiroshima Journal of Medical Sciences
  3. 49巻1号

Albumin Permeability Across Endothelial Cell Monolayer Exposed to Reactive Oxygen Intermediates : Involvement of Reversible Functional Alteration of the Cell Membrane Ca2+ Channels

https://hiroshima.repo.nii.ac.jp/records/2013478
https://hiroshima.repo.nii.ac.jp/records/2013478
f6e6d13e-fb2c-4209-9b9a-78ecb3a1f76f
名前 / ファイル ライセンス アクション
HiroshimaJMedSci_49_57.pdf HiroshimaJMedSci_49_57.pdf (899.2 KB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Albumin Permeability Across Endothelial Cell Monolayer Exposed to Reactive Oxygen Intermediates : Involvement of Reversible Functional Alteration of the Cell Membrane Ca2+ Channels
言語 en
作成者 Saeki, Noboru

× Saeki, Noboru

en Saeki, Noboru

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Az-Ma, Toshiharu

× Az-Ma, Toshiharu

en Az-Ma, Toshiharu

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Fujii, Kohyu

× Fujii, Kohyu

en Fujii, Kohyu

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Kawamoto, Masashi

× Kawamoto, Masashi

en Kawamoto, Masashi

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Yuge, Osafumi

× Yuge, Osafumi

en Yuge, Osafumi

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
主題
主題Scheme Other
主題 Albumin permeability
主題
主題Scheme Other
主題 Reactive oxygen intermediates
主題
主題Scheme Other
主題 Calcium channels
主題
主題Scheme Other
主題 Redox state
主題
主題Scheme NDC
主題 490
内容記述
内容記述 This study was designed to test the idea that the redox state of sulfhydryl (SH)-groups in cell-membrane Ca2+ channels plays a pivotal role in Ca2+ influx, which in turn causes an increase in albumin permeability across the cultured monolayer of porcine pulmonary artery endothelial (PPAE) cells exposed to xanthine/xanthine oxidase (X/XO). Albumin permeability as well as the concentration of intracellular Ca2+ ([Ca2+]i) was increased by X/XO. A H202 scavenger (catalase), an iron chelator (o-phenanthroline), and a hydroxyl radical scavenger (dimethyl sulfoxide) inhibited these changes provoked by X/XO, in which intracellular iron-catalyzed hydroxyl radical generation was suggested to be involved. The increase in albumin permeability and [Ca2+]i continued once the PPAE cells were exposed to X/XO. The [Ca2+]i was decreased by a Ca2+ channel blocker, Ni2+, while the removal of Ni2+ increased [Ca2+]i again, suggesting the sustained Ca2+ influx through cell-membrane Ca2+ channels was responsible for the [Ca2+]i elevation. Ni2+ failed to inhibit albumin permeability sustained after the removal of X/XO. In contrast, SH-reducing agents (dithiothreitol and glutathione) inhibited the sustained permeability as well as Ca2+ influx. We concluded that the redox alteration of SH-groups in cell-membrane Ca2+ channels was involved in the increase in albumin permeability after exposure of the endothelial cells to oxidative stress.
言語 en
内容記述
内容記述タイプ Other
内容記述 This study was supported in part by a Grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (No. 09771160).
出版者
出版者 Hiroshima University Medical Press
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ departmental bulletin paper
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0018-2052
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AA00664312
開始ページ
開始ページ 57
書誌情報 Hiroshima Journal of Medical Sciences
Hiroshima Journal of Medical Sciences

巻 49, 号 1, p. 57-65, 発行日 2000-03
旧ID 37702
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