{"created":"2025-02-23T06:34:31.048963+00:00","id":2013478,"links":{},"metadata":{"_buckets":{"deposit":"9671c5a4-b66a-409d-bb44-79e3c400e090"},"_deposit":{"created_by":41,"id":"2013478","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2013478"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02013478","sets":["1727147343679:1730442224523:1730442294845"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Albumin Permeability Across Endothelial Cell Monolayer Exposed to Reactive Oxygen Intermediates : Involvement of Reversible Functional Alteration of the Cell Membrane Ca2+ Channels","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Saeki, Noboru","creatorNameLang":"en"}],"familyNames":[{"familyName":"Saeki","familyNameLang":"en"}],"givenNames":[{"givenName":"Noboru","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Az-Ma, Toshiharu","creatorNameLang":"en"}],"familyNames":[{"familyName":"Az-Ma","familyNameLang":"en"}],"givenNames":[{"givenName":"Toshiharu","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Fujii, Kohyu","creatorNameLang":"en"}],"familyNames":[{"familyName":"Fujii","familyNameLang":"en"}],"givenNames":[{"givenName":"Kohyu","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kawamoto, Masashi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kawamoto","familyNameLang":"en"}],"givenNames":[{"givenName":"Masashi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yuge, Osafumi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yuge","familyNameLang":"en"}],"givenNames":[{"givenName":"Osafumi","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Albumin permeability","subitem_subject_scheme":"Other"},{"subitem_subject":"Reactive oxygen intermediates","subitem_subject_scheme":"Other"},{"subitem_subject":"Calcium channels","subitem_subject_scheme":"Other"},{"subitem_subject":"Redox state","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"This study was designed to test the idea that the redox state of sulfhydryl (SH)-groups in cell-membrane Ca2+ channels plays a pivotal role in Ca2+ influx, which in turn causes an increase in albumin permeability across the cultured monolayer of porcine pulmonary artery endothelial (PPAE) cells exposed to xanthine/xanthine oxidase (X/XO). Albumin permeability as well as the concentration of intracellular Ca2+ ([Ca2+]i) was increased by X/XO. A H202 scavenger (catalase), an iron chelator (o-phenanthroline), and a hydroxyl radical scavenger (dimethyl sulfoxide) inhibited these changes provoked by X/XO, in which intracellular iron-catalyzed hydroxyl radical generation was suggested to be involved. The increase in albumin permeability and [Ca2+]i continued once the PPAE cells were exposed to X/XO. The [Ca2+]i was decreased by a Ca2+ channel blocker, Ni2+, while the removal of Ni2+ increased [Ca2+]i again, suggesting the sustained Ca2+ influx through cell-membrane Ca2+ channels was responsible for the [Ca2+]i elevation. Ni2+ failed to inhibit albumin permeability sustained after the removal of X/XO. In contrast, SH-reducing agents (dithiothreitol and glutathione) inhibited the sustained permeability as well as Ca2+ influx. We concluded that the redox alteration of SH-groups in cell-membrane Ca2+ channels was involved in the increase in albumin permeability after exposure of the endothelial cells to oxidative stress.","subitem_description_language":"en"},{"subitem_description":"This study was supported in part by a Grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (No. 09771160).","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Hiroshima University Medical Press"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0018-2052","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00664312","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"開始ページ","attribute_value_mlt":[{"subitem_start_page":"57"}]},"item_1617187056579":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"65","bibliographicPageStart":"57","bibliographicVolumeNumber":"49","bibliographic_titles":[{"bibliographic_title":"Hiroshima Journal of Medical Sciences"},{"bibliographic_title":"Hiroshima Journal of Medical Sciences"}]}]},"item_1617258105262":{"attribute_name":"item_1617258105262","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"HiroshimaJMedSci_49_57.pdf","filesize":[{"value":"899.2 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2013478/files/HiroshimaJMedSci_49_57.pdf"},"version_id":"75776b6a-5db1-49d1-be5b-5113dd47e428"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"37702"},"item_title":"Albumin Permeability Across Endothelial Cell Monolayer Exposed to Reactive Oxygen Intermediates : Involvement of Reversible Functional Alteration of the Cell Membrane Ca2+ Channels","item_type_id":"40003","owner":"41","path":["1730442294845"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2013478","relation_version_is_last":true,"title":["Albumin Permeability Across Endothelial Cell Monolayer Exposed to Reactive Oxygen Intermediates : Involvement of Reversible Functional Alteration of the Cell Membrane Ca2+ Channels"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-06-02T03:12:34.521712+00:00"}