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A New Method to Detect Response of Lymphocytes to Alla-antigens
https://hiroshima.repo.nii.ac.jp/records/2013397
https://hiroshima.repo.nii.ac.jp/records/2013397cb2a6bb8-3c50-465e-b871-662a38445459
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
HiroshimaJMedSci_45_99.pdf (526.4 KB)
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| Item type | デフォルトアイテムタイプ_（フル）(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-03-18 | |||||||
| タイトル | ||||||||
| タイトル | A New Method to Detect Response of Lymphocytes to Alla-antigens | |||||||
| 言語 | en | |||||||
| 作成者 |
Okimoto, Tatsuya
× Okimoto, Tatsuya
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| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | MLC | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | IL-2 mRNA | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | RT-PCR | |||||||
| 主題 | ||||||||
| 主題Scheme | NDC | |||||||
| 主題 | 490 | |||||||
| 内容記述 | ||||||||
| 内容記述 | The mixed lymphocyte reaction (MLR) is the most commonly used method of investigating the immunological response of lymphocytes. However, this conventional technique requires the use of a radioisotope and is time consuming. Furthermore, there is a possibility that MLR does not reflect the response of each lymphocyte subset individually. The purpose of this study is to determine whether detection of IL-2 mRNA by reverse transcription polymerase chain reaction (RT-PCR) can be used as a new method of studying a precise response of PEL to allo-antigens. The study was conducted using lymphocytes from healthy volunteers, a renal transplant recipient who exhibited donor specific MLR unresponsiveness, the donor and the recipient's father. A kinetics study revealed that the expression of IL-2 mRNA in the MLC reached its maximum at 24-48 h. Lymphocytes from a renal transplant recipient also exhibited the same IL-2 mRNA expression pattern in response to stimulation with PEL from a non-related 3rd party but not to that from the donor. These data suggest that the expression level of IL-2 mRNA in MLC measured by RT-PCR at 24-48 h provides accurate response of PEL to allo-antigens. | |||||||
| 言語 | en | |||||||
| 出版者 | ||||||||
| 出版者 | Hiroshima University Medical Press | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0018-2052 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA00664312 | |||||||
| 開始ページ | ||||||||
| 開始ページ | 99 | |||||||
| 書誌情報 |
Hiroshima Journal of Medical Sciences Hiroshima Journal of Medical Sciences 巻 45, 号 3, p. 99-103, 発行日 1996-09 |
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| 旧ID | 37847 | |||||||
