{"created":"2025-02-23T06:32:07.912145+00:00","id":2013397,"links":{},"metadata":{"_buckets":{"deposit":"6fecfd07-67b6-4e53-b367-dedcadfb60d6"},"_deposit":{"created_by":41,"id":"2013397","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2013397"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02013397","sets":["1727147343679:1730442224523:1730442305026"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"A New Method to Detect Response of Lymphocytes to Alla-antigens","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Okimoto, Tatsuya","creatorNameLang":"en"}],"familyNames":[{"familyName":"Okimoto","familyNameLang":"en"}],"givenNames":[{"givenName":"Tatsuya","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"MLC","subitem_subject_scheme":"Other"},{"subitem_subject":"IL-2 mRNA","subitem_subject_scheme":"Other"},{"subitem_subject":"RT-PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"The mixed lymphocyte reaction (MLR) is the most commonly used method of investigating the immunological response of lymphocytes. However, this conventional technique requires the use of a radioisotope and is time consuming. Furthermore, there is a possibility that MLR does not reflect the response of each lymphocyte subset individually. The purpose of this study is to determine whether detection of IL-2 mRNA by reverse transcription polymerase chain reaction (RT-PCR) can be used as a new method of studying a precise response of PEL to allo-antigens. The study was conducted using lymphocytes from healthy volunteers, a renal transplant recipient who exhibited donor specific MLR unresponsiveness, the donor and the recipient's father. A kinetics study revealed that the expression of IL-2 mRNA in the MLC reached its maximum at 24-48 h. Lymphocytes from a renal transplant recipient also exhibited the same IL-2 mRNA expression pattern in response to stimulation with PEL from a non-related 3rd party but not to that from the donor. These data suggest that the expression level of IL-2 mRNA in MLC measured by RT-PCR at 24-48 h provides accurate response of PEL to allo-antigens.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Hiroshima University Medical Press"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0018-2052","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00664312","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"開始ページ","attribute_value_mlt":[{"subitem_start_page":"99"}]},"item_1617187056579":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"103","bibliographicPageStart":"99","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"Hiroshima Journal of Medical Sciences"},{"bibliographic_title":"Hiroshima Journal of Medical Sciences"}]}]},"item_1617258105262":{"attribute_name":"item_1617258105262","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"HiroshimaJMedSci_45_99.pdf","filesize":[{"value":"526.4 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2013397/files/HiroshimaJMedSci_45_99.pdf"},"version_id":"cab2f7d7-e941-4a64-9432-0f3ec84055e0"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"37847"},"item_title":"A New Method to Detect Response of Lymphocytes to Alla-antigens","item_type_id":"40003","owner":"41","path":["1730442305026"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2013397","relation_version_is_last":true,"title":["A New Method to Detect Response of Lymphocytes to Alla-antigens"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-06-02T03:08:30.941840+00:00"}