| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Production of Interleukin 2 in Human Peripheral Blood Lymphocytes: Optimal Condition for its Culture |
|
言語 |
en |
| 作成者 |
YAMAKIDO, Michio
YANAGIDA, Jitsuro
ISHIOKA, Sinichi
MATSUZAKA, Shigeru
HOZAWA, Soichiro
AKIYAMA, Mitoshi
KOBUKE, Kyoko
INAMIZU, Tsutomu
NISHIMOTO, Yukio
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Production of Interleukin 2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Human peripheral blood lymphocytes |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Optimal condition |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
CTLL-2 microassay |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
For the assay of the production of Interleukin 2 (IL-2) in human peripheral blood lymphocytes (human PBL), a study was made on the optimal condition for its culture. 1) The optimal condition for the production of IL-2 was considered to be incubation time of 24 hr, number of PBL of 1 x 106 cells/ml, and phytohemagglutinin-M (PHA-M) concentration of 1 — 5% 2) By one way mixed lymphocyte reaction (one way MLR), IL-2 activity similar in level to that by PHA-M stimulation could also be obtained. It was maximal at the 4th day of culture. 3) By PHA-P (0.06%) stimulation, IL-2 could also be produced similar to that by PHAM stimulation but it decreased in the order of Con A, PWM and PPD and hardly any production of IL-2 could be observed by LPS. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Hiroshima University School of Medicine |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
3874854 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0018-2052 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00664312 |
| 開始ページ |
|
|
開始ページ |
43 |
| 書誌情報 |
Hiroshima Journal of Medical Sciences
Hiroshima Journal of Medical Sciences
巻 34,
号 1,
p. 43-51,
発行日 1985-03
|
| 旧ID |
49832 |
HiroshimaJMedSci_34_43.pdf (1.1 MB)
