{"created":"2025-02-23T06:20:42.908556+00:00","id":2013001,"links":{},"metadata":{"_buckets":{"deposit":"8a96c998-e688-464d-a1bf-b1ee7399af41"},"_deposit":{"created_by":41,"id":"2013001","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2013001"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02013001","sets":["1727147343679:1730442224523:1730442338058"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Production of Interleukin 2 in Human Peripheral Blood Lymphocytes: Optimal Condition for its Culture","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"YAMAKIDO, Michio ","creatorNameLang":"en"}],"familyNames":[{"familyName":"YAMAKIDO","familyNameLang":"en"}],"givenNames":[{"givenName":"Michio ","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"YANAGIDA, Jitsuro","creatorNameLang":"en"}],"familyNames":[{"familyName":"YANAGIDA","familyNameLang":"en"}],"givenNames":[{"givenName":"Jitsuro","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"ISHIOKA, Sinichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"ISHIOKA","familyNameLang":"en"}],"givenNames":[{"givenName":"Sinichi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"MATSUZAKA, Shigeru","creatorNameLang":"en"}],"familyNames":[{"familyName":"MATSUZAKA","familyNameLang":"en"}],"givenNames":[{"givenName":"Shigeru","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"HOZAWA, Soichiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"HOZAWA","familyNameLang":"en"}],"givenNames":[{"givenName":"Soichiro","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"AKIYAMA, Mitoshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"AKIYAMA","familyNameLang":"en"}],"givenNames":[{"givenName":"Mitoshi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"KOBUKE, Kyoko","creatorNameLang":"en"}],"familyNames":[{"familyName":"KOBUKE","familyNameLang":"en"}],"givenNames":[{"givenName":"Kyoko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"INAMIZU, Tsutomu","creatorNameLang":"en"}],"familyNames":[{"familyName":"INAMIZU","familyNameLang":"en"}],"givenNames":[{"givenName":"Tsutomu","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"NISHIMOTO, Yukio","creatorNameLang":"en"}],"familyNames":[{"familyName":"NISHIMOTO","familyNameLang":"en"}],"givenNames":[{"givenName":"Yukio","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Production of Interleukin 2","subitem_subject_scheme":"Other"},{"subitem_subject":"Human peripheral blood lymphocytes","subitem_subject_scheme":"Other"},{"subitem_subject":"Optimal condition","subitem_subject_scheme":"Other"},{"subitem_subject":"CTLL-2 microassay","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"For the assay of the production of Interleukin 2 (IL-2) in human peripheral blood lymphocytes (human PBL), a study was made on the optimal condition for its culture.  1) The optimal condition for the production of IL-2 was considered to be incubation time of 24 hr, number of PBL of 1 x 106 cells/ml, and phytohemagglutinin-M (PHA-M) concentration of 1 — 5%  2) By one way mixed lymphocyte reaction (one way MLR), IL-2 activity similar in level to that by PHA-M stimulation could also be obtained. It was maximal at the 4th day of culture.  3) By PHA-P (0.06%) stimulation, IL-2 could also be produced similar to that by PHAM stimulation but it decreased in the order of Con A, PWM and PPD and hardly any production of IL-2 could be observed by LPS.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Hiroshima University School of Medicine"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0018-2052","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00664312","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"開始ページ","attribute_value_mlt":[{"subitem_start_page":"43"}]},"item_1617187056579":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1985-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"51","bibliographicPageStart":"43","bibliographicVolumeNumber":"34","bibliographic_titles":[{"bibliographic_title":"Hiroshima Journal of Medical Sciences"},{"bibliographic_title":"Hiroshima Journal of Medical Sciences"}]}]},"item_1617258105262":{"attribute_name":"item_1617258105262","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"3874854","subitem_relation_type_select":"PMID"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"HiroshimaJMedSci_34_43.pdf","filesize":[{"value":"1.1 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2013001/files/HiroshimaJMedSci_34_43.pdf"},"version_id":"97691a07-2dee-441e-afbc-b1648a6cb0ec"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"49832"},"item_title":"Production of Interleukin 2 in Human Peripheral Blood Lymphocytes: Optimal Condition for its Culture","item_type_id":"40003","owner":"41","path":["1730442338058"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2013001","relation_version_is_last":true,"title":["Production of Interleukin 2 in Human Peripheral Blood Lymphocytes: Optimal Condition for its Culture"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-06-02T02:54:21.442127+00:00"}