| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Characterization of rat and human CYP2J enzymes as Vitamin D 25-hydroxylases |
|
言語 |
en |
| 作成者 |
Aiba, Isamu
Yamasaki, Tomoaki
Shinki, Toshimasa
Izumi, Shunsuke
Yamamoto, Keiko
Yamada, Sachiko
Terato, Hiroaki
Ide, Hiroshi
Ohyama, Yoshihiko
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2006 Elsevier Inc. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
25-Hydroxylase |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
CYP2J2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
CYP2J3 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
vitamin D2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
vitamin D3 |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
vitamin D is 25-hydroxylated in the liver, before being activated by 1α-hydroxylation in the kidney. Recently, the rat cytochrome P450 2J3 (CYP2J3) has been identified as a principal vitamin D 25-hydroxylase in the rat [Yamasaki T, Izumi S, Ide H, Ohyama Y. Identification of a novel rat microsomal vitamin D3 25-hydroxylase. J Biol Chem 2004;279(22):22848-56]. In this study, we examine whether human CYP2J2 that exhibits 73 0x1.39bf8p-890mino acid homology to rat CYP2J3 has similar catalytic properties. Recombinant human CYP2J2 was overexpressed in Escherichia coli, purified, and assayed for vitamin D 25-hydroxylation activity. We found significant 25-hydroxylation activity toward vitamin D3 (turnover number, 0.087 min-1), vitamin D2 (0.16 min-1), and 1α-hydroxyvitamin D3 (2.2 min-1). Interestingly, human CYP2J2 hydroxylated vitamin D2, an exogenous vitamin D, at a higher rate than it did vitamin D3, an endogenous vitamin D, whereas, rat CYP2J3 hydroxylated vitamin D3 (1.4 min-1) more efficiently than vitamin D2 (0.86 min-1). Our study demonstrated that human CYP2J2 exhibits 25-hydroxylation activity as well as rat CYP2J3, although the activity of human CYP2J2 is weaker than rat CYP2J3. CYP2J2 and CYP2J3 exhibit distinct preferences toward vitamin D3 and D2. c 2006 Elsevier Inc. All rights reserved. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier Inc |
| 日付 |
|
|
日付 |
2006 |
|
日付タイプ |
Created |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.steroids.2006.04.009 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
16842832 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.steroids.2006.04.009 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0039-128X |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00850124 |
| 開始ページ |
|
|
開始ページ |
849 |
| 書誌情報 |
Steroids
Steroids
巻 71,
号 10,
p. 849-856,
発行日 2006
|
| 旧ID |
15353 |
Steroids_741_849.pdf (398.5 KB)
