{"created":"2025-02-21T04:36:28.930604+00:00","id":2008836,"links":{},"metadata":{"_buckets":{"deposit":"3a48843e-887a-45f2-8bb1-47105d0c8e09"},"_deposit":{"created_by":41,"id":"2008836","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2008836"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02008836","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Characterization of rat and human CYP2J enzymes as Vitamin D 25-hydroxylases","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Aiba, Isamu","creatorNameLang":"en"}],"familyNames":[{"familyName":"Aiba","familyNameLang":"en"}],"givenNames":[{"givenName":"Isamu","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamasaki, Tomoaki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yamasaki","familyNameLang":"en"}],"givenNames":[{"givenName":"Tomoaki","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shinki, Toshimasa","creatorNameLang":"en"}],"familyNames":[{"familyName":"Shinki","familyNameLang":"en"}],"givenNames":[{"givenName":"Toshimasa","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Izumi, Shunsuke","creatorNameLang":"en"}],"familyNames":[{"familyName":"Izumi","familyNameLang":"en"}],"givenNames":[{"givenName":"Shunsuke","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamamoto, Keiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yamamoto","familyNameLang":"en"}],"givenNames":[{"givenName":"Keiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamada, Sachiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yamada","familyNameLang":"en"}],"givenNames":[{"givenName":"Sachiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Terato, Hiroaki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Terato","familyNameLang":"en"}],"givenNames":[{"givenName":"Hiroaki","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ide, Hiroshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ide","familyNameLang":"en"}],"givenNames":[{"givenName":"Hiroshi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ohyama, Yoshihiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ohyama","familyNameLang":"en"}],"givenNames":[{"givenName":"Yoshihiko","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2006 Elsevier Inc."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"25-Hydroxylase","subitem_subject_scheme":"Other"},{"subitem_subject":"CYP2J2","subitem_subject_scheme":"Other"},{"subitem_subject":"CYP2J3","subitem_subject_scheme":"Other"},{"subitem_subject":"vitamin D2","subitem_subject_scheme":"Other"},{"subitem_subject":"vitamin D3","subitem_subject_scheme":"Other"},{"subitem_subject":"460","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"vitamin D is 25-hydroxylated in the liver, before being activated by 1α-hydroxylation in the kidney. Recently, the rat cytochrome P450 2J3 (CYP2J3) has been identified as a principal vitamin D 25-hydroxylase in the rat [Yamasaki T, Izumi S, Ide H, Ohyama Y. Identification of a novel rat microsomal vitamin D3 25-hydroxylase. J Biol Chem 2004;279(22):22848-56]. In this study, we examine whether human CYP2J2 that exhibits 73 0x1.39bf8p-890mino acid homology to rat CYP2J3 has similar catalytic properties. Recombinant human CYP2J2 was overexpressed in Escherichia coli, purified, and assayed for vitamin D 25-hydroxylation activity. We found significant 25-hydroxylation activity toward vitamin D3 (turnover number, 0.087 min-1), vitamin D2 (0.16 min-1), and 1α-hydroxyvitamin D3 (2.2 min-1). Interestingly, human CYP2J2 hydroxylated vitamin D2, an exogenous vitamin D, at a higher rate than it did vitamin D3, an endogenous vitamin D, whereas, rat CYP2J3 hydroxylated vitamin D3 (1.4 min-1) more efficiently than vitamin D2 (0.86 min-1). Our study demonstrated that human CYP2J2 exhibits 25-hydroxylation activity as well as rat CYP2J3, although the activity of human CYP2J2 is weaker than rat CYP2J3. CYP2J2 and CYP2J3 exhibit distinct preferences toward vitamin D3 and D2. c 2006 Elsevier Inc. All rights reserved.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc"}]},"item_1617186660861":{"attribute_name":"Date","attribute_value_mlt":[{"subitem_date_issued_datetime":"2006","subitem_date_issued_type":"Created"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0039-128X","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00850124","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"849"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"856","bibliographicPageStart":"849","bibliographicVolumeNumber":"71","bibliographic_titles":[{"bibliographic_title":"Steroids"},{"bibliographic_title":"Steroids"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.steroids.2006.04.009","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"16842832","subitem_relation_type_select":"PMID"}},{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.steroids.2006.04.009","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"Steroids_741_849.pdf","filesize":[{"value":"398.5 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2008836/files/Steroids_741_849.pdf"},"version_id":"7bee06e0-0093-485a-b651-39f63c3a5b8d"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"15353"},"item_title":"Characterization of rat and human CYP2J enzymes as Vitamin D 25-hydroxylases","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2008836","relation_version_is_last":true,"title":["Characterization of rat and human CYP2J enzymes as Vitamin D 25-hydroxylases"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-22T04:25:49.597068+00:00"}