| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
The integrin α5β1 regulates chondrocyte hypertrophic differentiation induced by GTP-bound transglutaminase 2 |
|
言語 |
en |
| 作成者 |
Tanaka, Kumi
Yokosaki, Yasuyuki
Higashikawa, Fumiko
Saito, Yuji
Eboshida, Akira
Ochi, Mitsuo
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2007 International Society of Matrix Biology Published by Elsevier B.V. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Integrin |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Transglutaminase 2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Chondrocyte |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Hypertrophy |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Differentiation |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
Soluble GTP-bound transglutaminase 2 (TG2) induces hypertrophic differentiation in chondrocyte cultures in a β1 integrin-dependent fashion. β1 integrin subfamily consists of 12 heterodimers with 12 different α subunits and a β1 subunit. To identify the specific integrin heterodimer(s) responsible for this process, we specifically blocked individual β1 integrins on the CH-8 immortalized human chondrocytes during hypertrophic differentiation. Blockade of α5β1 inhibited matrix metalloproteinase 13 (MMP-13), type X collagen expression, alkaline phosphatase activity, and matrix calcification by 30-50 %, associated with weak effects of anti-α3β1 and -α4β1. Anti-α1β1, -α2β1 and -α6β1 had no effect. To examine whether the dominant effect of integrin α5β1 was due to a direct interaction with TG2, we incubated the chondrocytic cells on plates coated with GTP-bound TG2. The immobilized GTP-bound TG2 induced hypertrophic differentiation to the same extent as the soluble GTP-bound TG2, which was also inhibited by anti-α5β1. CH-8 cells grown on plates coated with GTP-bound TG2 demonstrated adherence associated with focal adhesion kinase phosphorylation. These properties were inhibited by anti-α5β1. Furthermore, engagement of α5β1 on CH-8 cells via anti-α5β1 antibody did, in fact, induce differentiation. Although CH-8 cells adhered to GTP-free TG2 via integrin α5β1, the cells failed to undergo hypertrophic differentiation. Thus, integrin α5β1 is critical for the chondrocyte hypertrophic differentiation induced by GTP-bound TG2, and this induction is ligand-dependent. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier B.V. |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.matbio.2007.04.005 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.matbio.2007.04.005 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0945-053X |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10996308 |
| 開始ページ |
|
|
開始ページ |
409 |
| 書誌情報 |
Matrix Biology
Matrix Biology
巻 26,
号 2,
p. 409-418,
発行日 2007-06
|
| 旧ID |
20636 |
MatBio_26-2_409.pdf (406.3 KB)
