{"created":"2025-02-21T04:00:01.154370+00:00","id":2007690,"links":{},"metadata":{"_buckets":{"deposit":"02468f62-3c60-4dcf-9a2c-eedaa15dec5b"},"_deposit":{"created_by":41,"id":"2007690","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2007690"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02007690","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"The integrin α5β1 regulates chondrocyte hypertrophic differentiation induced by GTP-bound transglutaminase 2","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tanaka, Kumi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Tanaka","familyNameLang":"en"}],"givenNames":[{"givenName":"Kumi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yokosaki, Yasuyuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yokosaki","familyNameLang":"en"}],"givenNames":[{"givenName":"Yasuyuki","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Higashikawa, Fumiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Higashikawa","familyNameLang":"en"}],"givenNames":[{"givenName":"Fumiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Saito, Yuji","creatorNameLang":"en"}],"familyNames":[{"familyName":"Saito","familyNameLang":"en"}],"givenNames":[{"givenName":"Yuji","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Eboshida, Akira","creatorNameLang":"en"}],"familyNames":[{"familyName":"Eboshida","familyNameLang":"en"}],"givenNames":[{"givenName":"Akira","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ochi, Mitsuo","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ochi","familyNameLang":"en"}],"givenNames":[{"givenName":"Mitsuo","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2007 International Society of Matrix Biology Published by Elsevier B.V."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Integrin","subitem_subject_scheme":"Other"},{"subitem_subject":"Transglutaminase 2","subitem_subject_scheme":"Other"},{"subitem_subject":"Chondrocyte","subitem_subject_scheme":"Other"},{"subitem_subject":"Hypertrophy","subitem_subject_scheme":"Other"},{"subitem_subject":"Differentiation","subitem_subject_scheme":"Other"},{"subitem_subject":"460","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Soluble GTP-bound transglutaminase 2 (TG2) induces hypertrophic differentiation in chondrocyte cultures in a β1 integrin-dependent fashion. β1 integrin subfamily consists of 12 heterodimers with 12 different α subunits and a β1 subunit. To identify the specific integrin heterodimer(s) responsible for this process, we specifically blocked individual β1 integrins on the CH-8 immortalized human chondrocytes during hypertrophic differentiation. Blockade of α5β1 inhibited matrix metalloproteinase 13 (MMP-13), type X collagen expression, alkaline phosphatase activity, and matrix calcification by 30-50 %, associated with weak effects of anti-α3β1 and -α4β1. Anti-α1β1, -α2β1 and -α6β1 had no effect. To examine whether the dominant effect of integrin α5β1 was due to a direct interaction with TG2, we incubated the chondrocytic cells on plates coated with GTP-bound TG2. The immobilized GTP-bound TG2 induced hypertrophic differentiation to the same extent as the soluble GTP-bound TG2, which was also inhibited by anti-α5β1. CH-8 cells grown on plates coated with GTP-bound TG2 demonstrated adherence associated with focal adhesion kinase phosphorylation. These properties were inhibited by anti-α5β1. Furthermore, engagement of α5β1 on CH-8 cells via anti-α5β1 antibody did, in fact, induce differentiation. Although CH-8 cells adhered to GTP-free TG2 via integrin α5β1, the cells failed to undergo hypertrophic differentiation. Thus, integrin α5β1 is critical for the chondrocyte hypertrophic differentiation induced by GTP-bound TG2, and this induction is ligand-dependent.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0945-053X","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA10996308","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"409"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"418","bibliographicPageStart":"409","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"Matrix Biology"},{"bibliographic_title":"Matrix Biology"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.matbio.2007.04.005","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.matbio.2007.04.005","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"MatBio_26-2_409.pdf","filesize":[{"value":"406.3 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2007690/files/MatBio_26-2_409.pdf"},"version_id":"5a97fb53-7920-4362-92f7-5ca7c5329ebb"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"20636"},"item_title":"The integrin α5β1 regulates chondrocyte hypertrophic differentiation induced by GTP-bound transglutaminase 2","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2007690","relation_version_is_last":true,"title":["The integrin α5β1 regulates chondrocyte hypertrophic differentiation induced by GTP-bound transglutaminase 2"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T10:45:41.557907+00:00"}