| Item type |
デフォルトアイテムタイプ_(フル)(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Subunit C of the Vacuolar-Type ATPase from the Vanadium-Rich Ascidian, Ascidia sydneiensis samea, Rescued the pH Sensitivity of Yeast vma5 Mutants |
|
言語 |
en |
| 作成者 |
Ueki, Tatsuya
Uyama, Taro
Kanamori, Kan
Michibata, Hitoshi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2001 Springer "The original publication is available at www.springerlink.com" |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
ascidian |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
metal accumulation |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
vacuolar H+-ATPase |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
vacuolre |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
gene expression |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
A vanadium-accumulating ascidian, Ascidia sydneiensis samea, expresses vacuolar-type H+-ATPases (V ATPases) on the vacuole membrane of the vanadium-containing blood cells known as vanadocytes. Previously, we showed that the contents of their vacuoles are extremely acidic and that a V ATPase-specific inhibitor, bafilomycin A1, neutralized the contents of the vacuoles. To understand the function of V ATPase in vanadocytes, we isolated cDNA encoding subunit C of V ATPase from vanadocytes since this subunit has been known to be responsible for the assembly of V-ATPases and to regulate the ATPase activity of V-ATPases. The cloned cDNA was 1,443 nucleotides in length, and encoded a putative 384 amino-acid protein. By expressing the ascidian cDNA for subunit C under the control of a galactose-inducible promoter, the pH-sensitive phenotype of the corresponding vma5 mutant of a budding yeast was rescued. This result showed that the ascidian cDNA for subunit C functioned in yeast cells. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Springer |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1007/s1012601-0054-x |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
14961347 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1007/s1012601-0054-x |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1436-2228 |
| 開始ページ |
|
|
開始ページ |
316 |
| 書誌情報 |
Marine biotechnology
Marine biotechnology
巻 3,
p. 316-321,
発行日 2001
|
| 旧ID |
25 |