{"created":"2025-02-21T03:59:20.811117+00:00","id":2007668,"links":{},"metadata":{"_buckets":{"deposit":"d8c63a74-e358-4d55-ba97-1b66a01e2b6b"},"_deposit":{"created_by":41,"id":"2007668","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2007668"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02007668","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Subunit C of the Vacuolar-Type ATPase from the Vanadium-Rich Ascidian, Ascidia sydneiensis samea, Rescued the pH Sensitivity of Yeast vma5 Mutants","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ueki, Tatsuya","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ueki","familyNameLang":"en"}],"givenNames":[{"givenName":"Tatsuya","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Uyama, Taro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Uyama","familyNameLang":"en"}],"givenNames":[{"givenName":"Taro","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kanamori, Kan","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kanamori","familyNameLang":"en"}],"givenNames":[{"givenName":"Kan","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Michibata, Hitoshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Michibata","familyNameLang":"en"}],"givenNames":[{"givenName":"Hitoshi","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2001 Springer \"The original publication is available at www.springerlink.com\""}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"ascidian","subitem_subject_scheme":"Other"},{"subitem_subject":"metal accumulation","subitem_subject_scheme":"Other"},{"subitem_subject":"vacuolar H+-ATPase","subitem_subject_scheme":"Other"},{"subitem_subject":"vacuolre","subitem_subject_scheme":"Other"},{"subitem_subject":"gene expression","subitem_subject_scheme":"Other"},{"subitem_subject":"460","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"A vanadium-accumulating ascidian, Ascidia sydneiensis samea, expresses vacuolar-type H+-ATPases (V ATPases) on the vacuole membrane of the vanadium-containing blood cells known as vanadocytes. Previously, we showed that the contents of their vacuoles are extremely acidic and that a V ATPase-specific inhibitor, bafilomycin A1, neutralized the contents of the vacuoles. To understand the function of V ATPase in vanadocytes, we isolated cDNA encoding subunit C of V ATPase from vanadocytes since this subunit has been known to be responsible for the assembly of V-ATPases and to regulate the ATPase activity of V-ATPases. The cloned cDNA was 1,443 nucleotides in length, and encoded a putative 384 amino-acid protein. By expressing the ascidian cDNA for subunit C under the control of a galactose-inducible promoter, the pH-sensitive phenotype of the corresponding vma5 mutant of a budding yeast was rescued. This result showed that the ascidian cDNA for subunit C functioned in yeast cells.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Springer"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"1436-2228","subitem_source_identifier_type":"ISSN"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"316"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"321","bibliographicPageStart":"316","bibliographicVolumeNumber":"3","bibliographic_titles":[{"bibliographic_title":"Marine biotechnology"},{"bibliographic_title":"Marine biotechnology"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s1012601-0054-x","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"14961347","subitem_relation_type_select":"PMID"}},{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1007/s1012601-0054-x","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"MarineBiotech_3_316-321_2001.pdf","filesize":[{"value":"152.5 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2007668/files/MarineBiotech_3_316-321_2001.pdf"},"version_id":"90241d13-5805-47ca-bf6d-7be77e6d5d1c"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"25"},"item_title":"Subunit C of the Vacuolar-Type ATPase from the Vanadium-Rich Ascidian, Ascidia sydneiensis samea, Rescued the pH Sensitivity of Yeast vma5 Mutants","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2007668","relation_version_is_last":true,"title":["Subunit C of the Vacuolar-Type ATPase from the Vanadium-Rich Ascidian, Ascidia sydneiensis samea, Rescued the pH Sensitivity of Yeast vma5 Mutants"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T10:43:36.797347+00:00"}