| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Effects of endocytosis inhibitors on internalization of human IgG by Caco-2 human intestinal epithelial cells |
|
言語 |
en |
| 作成者 |
Sato, Koya
Nagai, Junya
Mitsui, Naoko
Yumoto, Ryoko
Takano, Mikihisa
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2009 Elsevier Inc. All rights reserved. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
IgG |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
FcRn |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Endocytosis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Caco-2 cells |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Intestine |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
Aims: The purpose of this study was to characterize the internalization mechanism of human IgG into the epithelial cells of human small intestine. employing human intestinal epithelial cell line Caco-2 as an in vitro model system. Main methods: Real-time PCR analysis and uptake studies of fluorescein isothiocyanate-labeled IgG (FITC-IgG) from human serum were performed using Caco-2 cells. Key findings: Real-time PCR analysis showed that mRNA level of the neonatal Fc receptor (FcRn) was increased during the differentiation process in Caco-2 cells. The binding of FITC-labeled human IgG to the membrane surface of Caco-2 cells increased with a decrease in pH of incubation buffer. The uptake of FITC-IgG was also stimulated at acidic pH and was time-dependent. The binding and uptake of FITC-IgG at pH 6.0 was partially, but significantly, decreased by human gamma-globulin in a concentration-dependent manner. A mixture of metabolic inhibitors (sodium azide and 2-deoxyglucose) significantly inhibited the uptake, but not the binding, of FITC-IgG. In addition, endosomal acidification inhibitors such as bafilomycin A, and chloroquine significantly increased the accumulation of FITC-IgG. Clathrin-dependent endocytosis inhibitors (phenylarsine oxide and chlorpromazine) and caveolin-dependent endocytosis inhibitors (nystatin and indomethacin) did not decrease the uptake of FITC-IgG at pH 6.0. In contrast, macropinocytosis inhibitors such as cytochalasin B and 5-(N-ethyl-N-isopropyl) amiloride significantly decreased the uptake of FITC-IgG at pH 6.0. Significance: The internalization of human IgG in human intestine might be, at least in part, due to FcRn-mediated endocytosis. which could occur by a process other than clathrin- and caveolin-dependent mechanisms. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Pergamon |
| 出版者 |
|
|
出版者 |
Elsevier Science Ltd |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.lfs.2009.10.012 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.lfs.2009.10.012 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0024-3205 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00717011 |
| 開始ページ |
|
|
開始ページ |
800 |
| 書誌情報 |
Life Sciences
Life Sciences
巻 85,
号 23-26,
p. 800-807,
発行日 2009-12-16
|
| 旧ID |
29237 |
LifeSci_85_800.pdf (530.9 KB)
