{"created":"2025-02-21T03:58:42.779181+00:00","id":2007647,"links":{},"metadata":{"_buckets":{"deposit":"232c9fb4-882f-4562-af4e-303ee1fda8c0"},"_deposit":{"created_by":41,"id":"2007647","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2007647"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02007647","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Effects of endocytosis inhibitors on internalization of human IgG by Caco-2 human intestinal epithelial cells","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sato, Koya","creatorNameLang":"en"}],"familyNames":[{"familyName":"Sato","familyNameLang":"en"}],"givenNames":[{"givenName":"Koya","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nagai, Junya","creatorNameLang":"en"}],"familyNames":[{"familyName":"Nagai","familyNameLang":"en"}],"givenNames":[{"givenName":"Junya","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mitsui, Naoko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Mitsui","familyNameLang":"en"}],"givenNames":[{"givenName":"Naoko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yumoto, Ryoko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yumoto","familyNameLang":"en"}],"givenNames":[{"givenName":"Ryoko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takano, Mikihisa","creatorNameLang":"en"}],"familyNames":[{"familyName":"Takano","familyNameLang":"en"}],"givenNames":[{"givenName":"Mikihisa","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2009 Elsevier Inc. All rights reserved."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"IgG","subitem_subject_scheme":"Other"},{"subitem_subject":"FcRn","subitem_subject_scheme":"Other"},{"subitem_subject":"Endocytosis","subitem_subject_scheme":"Other"},{"subitem_subject":"Caco-2 cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Intestine","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Aims: The purpose of this study was to characterize the internalization mechanism of human IgG into the epithelial cells of human small intestine. employing human intestinal epithelial cell line Caco-2 as an in vitro model system.    Main methods: Real-time PCR analysis and uptake studies of fluorescein isothiocyanate-labeled IgG (FITC-IgG) from human serum were performed using Caco-2 cells.    Key findings: Real-time PCR analysis showed that mRNA level of the neonatal Fc receptor (FcRn) was increased during the differentiation process in Caco-2 cells. The binding of FITC-labeled human IgG to the membrane surface of Caco-2 cells increased with a decrease in pH of incubation buffer. The uptake of FITC-IgG was also stimulated at acidic pH and was time-dependent. The binding and uptake of FITC-IgG at pH 6.0 was partially, but significantly, decreased by human gamma-globulin in a concentration-dependent manner. A mixture of metabolic inhibitors (sodium azide and 2-deoxyglucose) significantly inhibited the uptake, but not the binding, of FITC-IgG. In addition, endosomal acidification inhibitors such as bafilomycin A, and chloroquine significantly increased the accumulation of FITC-IgG. Clathrin-dependent endocytosis inhibitors (phenylarsine oxide and chlorpromazine) and caveolin-dependent endocytosis inhibitors (nystatin and indomethacin) did not decrease the uptake of FITC-IgG at pH 6.0. In contrast, macropinocytosis inhibitors such as cytochalasin B and 5-(N-ethyl-N-isopropyl) amiloride significantly decreased the uptake of FITC-IgG at pH 6.0.    Significance: The internalization of human IgG in human intestine might be, at least in part, due to FcRn-mediated endocytosis. which could occur by a process other than clathrin- and caveolin-dependent mechanisms.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Pergamon"},{"subitem_publisher":"Elsevier Science Ltd"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0024-3205","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00717011","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"800"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-12-16","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"23-26","bibliographicPageEnd":"807","bibliographicPageStart":"800","bibliographicVolumeNumber":"85","bibliographic_titles":[{"bibliographic_title":"Life Sciences"},{"bibliographic_title":"Life Sciences"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.lfs.2009.10.012","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.lfs.2009.10.012","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"LifeSci_85_800.pdf","filesize":[{"value":"530.9 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2007647/files/LifeSci_85_800.pdf"},"version_id":"cf7bb96c-8432-49e2-a7e1-15066f9db786"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"29237"},"item_title":"Effects of endocytosis inhibitors on internalization of human IgG by Caco-2 human intestinal epithelial cells","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2007647","relation_version_is_last":true,"title":["Effects of endocytosis inhibitors on internalization of human IgG by Caco-2 human intestinal epithelial cells"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T10:34:50.036292+00:00"}