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Basic Fibroblast Growth Factor Induces The Expression of Matrix Metalloproteinase-3 in Human Periodontal Ligament Cells Through the MEK2 Mitogen-Activated Protein Kinase Pathway
https://hiroshima.repo.nii.ac.jp/records/2007460
https://hiroshima.repo.nii.ac.jp/records/2007460bfb5dbcc-cc55-4e12-9a07-3ade62e9a44e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
JPR_38_122.pdf (1.4 MB)
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| Item type | デフォルトアイテムタイプ_（フル）(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-03-18 | |||||||||
| タイトル | ||||||||||
| タイトル | Basic Fibroblast Growth Factor Induces The Expression of Matrix Metalloproteinase-3 in Human Periodontal Ligament Cells Through the MEK2 Mitogen-Activated Protein Kinase Pathway | |||||||||
| 言語 | en | |||||||||
| 作成者 |
Shimazu, Atsushi
× Shimazu, Atsushi
× Morishita, Masayuki
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| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 権利情報 | ||||||||||
| 権利情報 | Copyright (c) 2003 Blackwell Publishing. The definitive version is available at www.blackwell-synergy.com | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | periodontal ligament (PDL) | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | basic fibroblast growth factor (bFGF, FGF-2) | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | matrix metalloproteinase (MMP) -3 | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | mitogen-activated protein (MAP) kinase | |||||||||
| 主題 | ||||||||||
| 主題Scheme | NDC | |||||||||
| 主題 | 490 | |||||||||
| 内容記述 | ||||||||||
| 内容記述 | Basic fibroblast growth factor (bFGF, FGF-2) is one of the potent mitogens for periodontal ligament (PDL) cells. However, the role of bFGF on the matrix metalloproteinase-3 (MMP-3) expression in PDL cells is unknown. In this study, the effect of bFGF on MMP-3 expression in PDL cells and the mechanism of this process were examined. Human PDL cells were exposed to bFGF at various concentrations (0.01–10 ng/ml) in monolayer cultures. bFGF increased [3H]thymidine incorporation and suppressed proteoglycan synthesis concentration-dependently. However, similar concentration ranges of bFGF increased the release of the cell-associated proteoglycans into the medium. Furthermore, bFGF increased MMP-3 mRNA levels concentration-dependently as examined by reverse transcription-polymerase chain reaction (RT-PCR). Induction of MMP-3 after the stimulation with bFGF was observed as early as 12 h with maximal at 24 h. Thereafter, the MMP-3 mRNA level gradually decreased until 72 h. Cycloheximide blocked the induction of MMP-3 by bFGF, indicating the requirement of de novo protein synthesis for this stimulation. Furthermore, MMP-3 expression induced by bFGF was abrogated by U0126, a specific inhibitor of MEK1/2 and ERK1/2 in mitogen-activated protein (MAP) kinase pathway, not by PD98059, a specific inhibitor of MEK1. In addition, bFGF up-regulated the phosphorylated ERK1/2 in 5 min with the maximal at 20 min as examined by Western blotting, and U0126 inhibited the ERK1/2 phosphorylation induced by bFGF. These findings suggest that bFGF induces MMP-3 expression in PDL cells through the activation of the MEK2 in MAP kinase pathway. bFGF stimulation on MMP-3 synthesis may be involved in the control of the cell-associated proteoglycans in PDL cells during periodontal regeneration and degradation. | |||||||||
| 言語 | en | |||||||||
| 出版者 | ||||||||||
| 出版者 | Blackwell | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AO | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_b1a7d7d4d402bcce | |||||||||
| 関連情報 | ||||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1034/j.1600-0765.2003.01645.x | |||||||||
| 関連情報 | ||||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | http://dx.doi.org/10.1034/j.1600-0765.2003.01645.x | |||||||||
| 収録物識別子 | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0022-3484 | |||||||||
| 収録物識別子 | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AA00704381 | |||||||||
| 開始ページ | ||||||||||
| 開始ページ | 122 | |||||||||
| 書誌情報 |
Journal of Periodontal Research Journal of Periodontal Research 巻 38, 号 2, p. 122-129, 発行日 2003 |
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| 旧ID | 21547 | |||||||||
