{"created":"2025-02-21T03:52:39.358943+00:00","id":2007460,"links":{},"metadata":{"_buckets":{"deposit":"16b0aaab-c289-4c53-84a8-e18233c33209"},"_deposit":{"created_by":41,"id":"2007460","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2007460"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02007460","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Basic Fibroblast Growth Factor Induces The Expression of Matrix Metalloproteinase-3 in Human Periodontal Ligament Cells Through the MEK2 Mitogen-Activated Protein Kinase Pathway","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shimazu, Atsushi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Shimazu","familyNameLang":"en"}],"givenNames":[{"givenName":"Atsushi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Morishita, Masayuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Morishita","familyNameLang":"en"}],"givenNames":[{"givenName":"Masayuki","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2003 Blackwell Publishing. The definitive version is available at www.blackwell-synergy.com"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"periodontal ligament (PDL)","subitem_subject_scheme":"Other"},{"subitem_subject":"basic fibroblast growth factor (bFGF, FGF-2)","subitem_subject_scheme":"Other"},{"subitem_subject":"matrix metalloproteinase (MMP) -3","subitem_subject_scheme":"Other"},{"subitem_subject":"mitogen-activated protein (MAP) kinase","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Basic fibroblast growth factor (bFGF, FGF-2) is one of the potent mitogens for periodontal ligament (PDL) cells. However, the role of bFGF on the matrix metalloproteinase-3 (MMP-3) expression in PDL cells is unknown. In this study, the effect of bFGF on MMP-3 expression in PDL cells and the mechanism of this process were examined. Human PDL cells were exposed to bFGF at various concentrations (0.01–10 ng/ml) in monolayer cultures. bFGF increased [3H]thymidine incorporation and suppressed proteoglycan synthesis concentration-dependently. However, similar concentration ranges of bFGF increased the release of the cell-associated proteoglycans into the medium. Furthermore, bFGF increased MMP-3 mRNA levels concentration-dependently as examined by reverse transcription-polymerase chain reaction (RT-PCR). Induction of MMP-3 after the stimulation with bFGF was observed as early as 12 h with maximal at 24 h. Thereafter, the MMP-3 mRNA level gradually decreased until 72 h. Cycloheximide blocked the induction of MMP-3 by bFGF, indicating the requirement of de novo protein synthesis for this stimulation. Furthermore, MMP-3 expression induced by bFGF was abrogated by U0126, a specific inhibitor of MEK1/2 and ERK1/2 in mitogen-activated protein (MAP) kinase pathway, not by PD98059, a specific inhibitor of MEK1. In addition, bFGF up-regulated the phosphorylated ERK1/2 in 5 min with the maximal at 20 min as examined by Western blotting, and U0126 inhibited the ERK1/2 phosphorylation induced by bFGF. These findings suggest that bFGF induces MMP-3 expression in PDL cells through the activation of the MEK2 in MAP kinase pathway. bFGF stimulation on MMP-3 synthesis may be involved in the control of the cell-associated proteoglycans in PDL cells during periodontal regeneration and degradation.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Blackwell"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0022-3484","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00704381","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"122"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"129","bibliographicPageStart":"122","bibliographicVolumeNumber":"38","bibliographic_titles":[{"bibliographic_title":"Journal of Periodontal Research"},{"bibliographic_title":"Journal of Periodontal Research"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1034/j.1600-0765.2003.01645.x","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1034/j.1600-0765.2003.01645.x","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"JPR_38_122.pdf","filesize":[{"value":"1.4 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2007460/files/JPR_38_122.pdf"},"version_id":"ea304a21-2916-4b58-9f01-5be5d9604a94"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"21547"},"item_title":"Basic Fibroblast Growth Factor Induces The Expression of Matrix Metalloproteinase-3 in Human Periodontal Ligament Cells Through the MEK2 Mitogen-Activated Protein Kinase Pathway","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2007460","relation_version_is_last":true,"title":["Basic Fibroblast Growth Factor Induces The Expression of Matrix Metalloproteinase-3 in Human Periodontal Ligament Cells Through the MEK2 Mitogen-Activated Protein Kinase Pathway"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T09:59:29.491206+00:00"}