| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Detection of single-copy functional genes in prokaryotic cells by two-pass TSA-FISH with polynucleotide probes |
|
言語 |
en |
| 作成者 |
Kawakami, Shuji
Hasegawa, Takuya
Imachi, Hiroyuki
Yamaguchi, Takashi
Harada, Hideki
Ohashi, Akiyoshi
Kubota, Kengo
|
| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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|
権利情報 |
(c) 2012 Elsevier B.V. All rights reserved. |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
Functional genes |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
In situ whole-cell detection |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Polynucleotide probes |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Two-pass TSA-FISH |
| 主題 |
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|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
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|
内容記述 |
In situ detection of functional genes with single-cell resolution is currently of interest to microbiologists. Here, we developed a two-pass tyramide signal amplification (TSA)-fluorescence in situ hybridization (FISH) protocol with PCR-derived polynucleotide probes for the detection of single-copy genes in prokaryotic cells. The mcrA gene and the apsA gene in methanogens and sulfate-reducing bacteria, respectively, were targeted. The protocol showed bright fluorescence with a good signal-to-noise ratio and achieved a high efficiency of detection (>98%). The discrimination threshold was approximately 82-89% sequence identity. Microorganisms possessing the mcrA or apsA gene in anaerobic sludge samples were successfully detected by two-pass TSA-FISH with polynucleotide probes. The developed protocol is useful for identifying single microbial cells based on functional gene sequences. |
|
言語 |
en |
| 出版者 |
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|
出版者 |
Elsevier Science BV |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.mimet.2011.11.014 |
| 関連情報 |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.mimet.2011.11.014 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0167-7012 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA1063956X |
| 開始ページ |
|
|
開始ページ |
218 |
| 書誌情報 |
Journal of Microbiological Methods
Journal of Microbiological Methods
巻 88,
号 2,
p. 218-223,
発行日 2012
|
| 旧ID |
34820 |
JMicrobioMethod_88_218.pdf (576.2 KB)
