{"created":"2025-02-21T03:50:54.424757+00:00","id":2007402,"links":{},"metadata":{"_buckets":{"deposit":"699e6c0a-12b1-4d8e-8434-4d5f39b5ec71"},"_deposit":{"created_by":41,"id":"2007402","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2007402"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02007402","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Detection of single-copy functional genes in prokaryotic cells by two-pass TSA-FISH with polynucleotide probes","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawakami, Shuji","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kawakami","familyNameLang":"en"}],"givenNames":[{"givenName":"Shuji","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hasegawa, Takuya","creatorNameLang":"en"}],"familyNames":[{"familyName":"Hasegawa","familyNameLang":"en"}],"givenNames":[{"givenName":"Takuya","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Imachi, Hiroyuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Imachi","familyNameLang":"en"}],"givenNames":[{"givenName":"Hiroyuki","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamaguchi, Takashi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yamaguchi","familyNameLang":"en"}],"givenNames":[{"givenName":"Takashi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Harada, Hideki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Harada","familyNameLang":"en"}],"givenNames":[{"givenName":"Hideki","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ohashi, Akiyoshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ohashi","familyNameLang":"en"}],"givenNames":[{"givenName":"Akiyoshi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kubota, Kengo","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kubota","familyNameLang":"en"}],"givenNames":[{"givenName":"Kengo","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"(c) 2012 Elsevier B.V. All rights reserved."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Functional genes","subitem_subject_scheme":"Other"},{"subitem_subject":"In situ whole-cell detection","subitem_subject_scheme":"Other"},{"subitem_subject":"Polynucleotide probes","subitem_subject_scheme":"Other"},{"subitem_subject":"Two-pass TSA-FISH","subitem_subject_scheme":"Other"},{"subitem_subject":"460","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"In situ detection of functional genes with single-cell resolution is currently of interest to microbiologists. Here, we developed a two-pass tyramide signal amplification (TSA)-fluorescence in situ hybridization (FISH) protocol with PCR-derived polynucleotide probes for the detection of single-copy genes in prokaryotic cells. The mcrA gene and the apsA gene in methanogens and sulfate-reducing bacteria, respectively, were targeted. The protocol showed bright fluorescence with a good signal-to-noise ratio and achieved a high efficiency of detection (>98%). The discrimination threshold was approximately 82-89% sequence identity. Microorganisms possessing the mcrA or apsA gene in anaerobic sludge samples were successfully detected by two-pass TSA-FISH with polynucleotide probes. The developed protocol is useful for identifying single microbial cells based on functional gene sequences.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier Science BV"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0167-7012","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA1063956X","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"218"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"223","bibliographicPageStart":"218","bibliographicVolumeNumber":"88","bibliographic_titles":[{"bibliographic_title":"Journal of Microbiological Methods"},{"bibliographic_title":"Journal of Microbiological Methods"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.mimet.2011.11.014","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.mimet.2011.11.014","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"JMicrobioMethod_88_218.pdf","filesize":[{"value":"576.2 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2007402/files/JMicrobioMethod_88_218.pdf"},"version_id":"266a2f86-c2f5-464d-8f4c-f0424f9234e1"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"34820"},"item_title":"Detection of single-copy functional genes in prokaryotic cells by two-pass TSA-FISH with polynucleotide probes","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2007402","relation_version_is_last":true,"title":["Detection of single-copy functional genes in prokaryotic cells by two-pass TSA-FISH with polynucleotide probes"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T09:53:47.692327+00:00"}