| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
High-sensitive analysis of DNA fragments by capillary gel electrophoresis using transient isotachophoresis preconcentration and fluorescence detection |
|
言語 |
en |
| 作成者 |
Xu, Zhongqi
Esumi, Toshiaki
Ikuta, Natsuki
Hirokawa, Takeshi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2009 Elsevier B.V. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Capillary gel electrophoresis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
DNA fragments |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Transient isotachophoresis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Fluorescence detection |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Ethidium bromide |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
570 |
| 内容記述 |
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|
内容記述 |
In this report aimed on further development of a high-sensitivity capillary gel electrophoresis (CCE) method for analysis of DNA fragments, we firstly explored online transient isotachophoresis (tITP) preconcentration combined with fluorescence detection (FD). The fluorescence signal (excitation: 488 nm; emission: 590 nm) was generated using the intercalating dye of ethidium bromide (EB). It was found when the leading electrolyte (LE) was injected behind the sample zone, such a special tITP mode has significant advantages to solve the bubble formation issue and to improve the analytical performance stability. Two standard DNA samples, a 50 bp DNA step ladder and the phi X174/HaeIII digest. were used to evaluate the qualitative and quantitative abilities of the tITP-FD approach. A highly diluted sample (10,000-fold in the water, e.g. the phi X174/HaeIII digest diluted from 500 mu g/ml to the 50 ng/ml level) was enriched and detected; the LOD was down to 0.09 ng/ml for the 72 bp fragment, apparently improved more than 1000-fold in comparison with UV detection. Although the RSD of peak areas (n = 3) was around 15.5% for the sample was electrokinetically injected, good linearity of peak area response showed that the proposed method is suitable for quantitative analysis. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier Science BV |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.chroma.2009.01.066 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.chroma.2009.01.066 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0021-9673 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00695371 |
| 開始ページ |
|
|
開始ページ |
3602 |
| 書誌情報 |
Journal of Chromatography A
Journal of Chromatography A
巻 1216,
号 17,
p. 3602-3605,
発行日 2009
|
| 旧ID |
27705 |
JChromA_1216_3602.pdf (238.7 KB)
