{"created":"2025-02-21T03:47:27.245685+00:00","id":2007289,"links":{},"metadata":{"_buckets":{"deposit":"ee31fa84-5c12-4053-95b5-77acec8aea03"},"_deposit":{"created_by":41,"id":"2007289","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2007289"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02007289","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"High-sensitive analysis of DNA fragments by capillary gel electrophoresis using transient isotachophoresis preconcentration and fluorescence detection","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Xu, Zhongqi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Xu","familyNameLang":"en"}],"givenNames":[{"givenName":"Zhongqi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Esumi, Toshiaki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Esumi","familyNameLang":"en"}],"givenNames":[{"givenName":"Toshiaki","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ikuta, Natsuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ikuta","familyNameLang":"en"}],"givenNames":[{"givenName":"Natsuki","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hirokawa, Takeshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Hirokawa","familyNameLang":"en"}],"givenNames":[{"givenName":"Takeshi","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2009 Elsevier B.V."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Capillary gel electrophoresis","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA fragments","subitem_subject_scheme":"Other"},{"subitem_subject":"Transient isotachophoresis","subitem_subject_scheme":"Other"},{"subitem_subject":"Fluorescence detection","subitem_subject_scheme":"Other"},{"subitem_subject":"Ethidium bromide","subitem_subject_scheme":"Other"},{"subitem_subject":"570","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"In this report aimed on further development of a high-sensitivity capillary gel electrophoresis (CCE) method for analysis of DNA fragments, we firstly explored online transient isotachophoresis (tITP) preconcentration combined with fluorescence detection (FD). The fluorescence signal (excitation: 488 nm; emission: 590 nm) was generated using the intercalating dye of ethidium bromide (EB). It was found when the leading electrolyte (LE) was injected behind the sample zone, such a special tITP mode has significant advantages to solve the bubble formation issue and to improve the analytical performance stability. Two standard DNA samples, a 50 bp DNA step ladder and the phi X174/HaeIII digest. were used to evaluate the qualitative and quantitative abilities of the tITP-FD approach. A highly diluted sample (10,000-fold in the water, e.g. the phi X174/HaeIII digest diluted from 500 mu g/ml to the 50 ng/ml level) was enriched and detected; the LOD was down to 0.09 ng/ml for the 72 bp fragment, apparently improved more than 1000-fold in comparison with UV detection. Although the RSD of peak areas (n = 3) was around 15.5% for the sample was electrokinetically injected, good linearity of peak area response showed that the proposed method is suitable for quantitative analysis.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier Science BV"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0021-9673","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00695371","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"3602"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"17","bibliographicPageEnd":"3605","bibliographicPageStart":"3602","bibliographicVolumeNumber":"1216","bibliographic_titles":[{"bibliographic_title":"Journal of Chromatography A"},{"bibliographic_title":"Journal of Chromatography A"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.chroma.2009.01.066","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.chroma.2009.01.066","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"JChromA_1216_3602.pdf","filesize":[{"value":"238.7 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2007289/files/JChromA_1216_3602.pdf"},"version_id":"b2fa62ba-5912-43b3-8601-a864d478db15"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"27705"},"item_title":"High-sensitive analysis of DNA fragments by capillary gel electrophoresis using transient isotachophoresis preconcentration and fluorescence detection","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2007289","relation_version_is_last":true,"title":["High-sensitive analysis of DNA fragments by capillary gel electrophoresis using transient isotachophoresis preconcentration and fluorescence detection"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T09:37:47.075215+00:00"}