| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
2-Aminopurine inhibits leptin receptor signal transduction |
|
言語 |
en |
| 作成者 |
Hosoi, Toru
Matsunami, Naomi
Nagahama, Tomoko
Okuma, Yasunobu
Ozawa, Koichiro
Takizawa, Tsuyoshi
Nomura, Yasuyuki
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2006 Elsevier Ltd. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
leptin |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
2-aminopurine |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
signal transducer and activator of transcription 3 (STAT3) |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
extracellular signal-regulated kinase (ERK) |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
double-strand RNA-activated protein kinase (PKR) |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Ob-Rb leptin receptor |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
Leptin is an important circulating signal for regulation of food intake and body weight. In the present study, we investigated the effect of 2-aminopurine (2-AP), an inhibitor of double-strand RNA-activated protein kinase (PKR), on leptin signal transduction. 2-AP dose-dependently inhibited leptin-induced phosporylation of signal transducer and activator of transcription 3 (STAT3), extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) in HEK293 cells stably transfected with the Ob-Rb leptin receptor. On the other hand, we observed only slight inhibition of leptin-induced STAT3 activation by purine treatment, indicating that the inhibitory effect will be dramatically enhanced in the presence of an amino group. 2-AP did not inhibit PMA-induced ERK activation, indicating that the effect may be leptin-signal specific. The inhibitory effect of 2-AP was not mediated by newly synthesized protein because the inhibitory effect of 2-AP on leptin-induced STAT3 activation was not abrogated in the presence of the protein synthesis inhibitor cycloheximide. Interestingly, leptin did not induce PKR activation, suggesting that the effect of 2-AP on leptin signal may be independent of PKR. Finally, 2-AP inhibited leptin-induced phosphorylation of the Ob-Rb leptin receptor. These results provide evidence of a novel action of 2-AP, i.e., inhibition of activation of leptin signal transduction at the level of the Ob-Rb leptin receptor. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.ejphar.2006.09.044 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
17070518 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.ejphar.2006.09.044 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0014-2999 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00639687 |
| 開始ページ |
|
|
開始ページ |
61 |
| 書誌情報 |
European Journal of Pharmacology
European Journal of Pharmacology
巻 553,
号 1-3,
p. 61-66,
発行日 2006-12-28
|
| 旧ID |
17066 |
EurJPharm_553_61.pdf (1.8 MB)
