{"created":"2025-02-21T03:33:47.939017+00:00","id":2006849,"links":{},"metadata":{"_buckets":{"deposit":"f97e1a8c-eb64-4917-ad81-168db9d777d4"},"_deposit":{"created_by":41,"id":"2006849","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006849"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006849","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"2-Aminopurine inhibits leptin receptor signal transduction","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hosoi, Toru","creatorNameLang":"en"}],"familyNames":[{"familyName":"Hosoi","familyNameLang":"en"}],"givenNames":[{"givenName":"Toru","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Matsunami, Naomi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Matsunami","familyNameLang":"en"}],"givenNames":[{"givenName":"Naomi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nagahama, Tomoko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Nagahama","familyNameLang":"en"}],"givenNames":[{"givenName":"Tomoko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Okuma, Yasunobu","creatorNameLang":"en"}],"familyNames":[{"familyName":"Okuma","familyNameLang":"en"}],"givenNames":[{"givenName":"Yasunobu","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ozawa, Koichiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ozawa","familyNameLang":"en"}],"givenNames":[{"givenName":"Koichiro","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takizawa, Tsuyoshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Takizawa","familyNameLang":"en"}],"givenNames":[{"givenName":"Tsuyoshi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nomura, Yasuyuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Nomura","familyNameLang":"en"}],"givenNames":[{"givenName":"Yasuyuki","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2006 Elsevier Ltd."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"leptin","subitem_subject_scheme":"Other"},{"subitem_subject":"2-aminopurine","subitem_subject_scheme":"Other"},{"subitem_subject":"signal transducer and activator of transcription 3 (STAT3)","subitem_subject_scheme":"Other"},{"subitem_subject":"extracellular signal-regulated kinase (ERK)","subitem_subject_scheme":"Other"},{"subitem_subject":"double-strand RNA-activated protein kinase (PKR)","subitem_subject_scheme":"Other"},{"subitem_subject":"Ob-Rb leptin receptor","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Leptin is an important circulating signal for regulation of food intake and body weight. In the present study, we investigated the effect of 2-aminopurine (2-AP), an inhibitor of double-strand RNA-activated protein kinase (PKR), on leptin signal transduction. 2-AP dose-dependently inhibited leptin-induced phosporylation of signal transducer and activator of transcription 3 (STAT3), extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) in HEK293 cells stably transfected with the Ob-Rb leptin receptor. On the other hand, we observed only slight inhibition of leptin-induced STAT3 activation by purine treatment, indicating that the inhibitory effect will be dramatically enhanced in the presence of an amino group. 2-AP did not inhibit PMA-induced ERK activation, indicating that the effect may be leptin-signal specific. The inhibitory effect of 2-AP was not mediated by newly synthesized protein because the inhibitory effect of 2-AP on leptin-induced STAT3 activation was not abrogated in the presence of the protein synthesis inhibitor cycloheximide. Interestingly, leptin did not induce PKR activation, suggesting that the effect of 2-AP on leptin signal may be independent of PKR. Finally, 2-AP inhibited leptin-induced phosphorylation of the Ob-Rb leptin receptor. These results provide evidence of a novel action of 2-AP, i.e., inhibition of activation of leptin signal transduction at the level of the Ob-Rb leptin receptor.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0014-2999","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00639687","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"61"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-12-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-3","bibliographicPageEnd":"66","bibliographicPageStart":"61","bibliographicVolumeNumber":"553","bibliographic_titles":[{"bibliographic_title":"European Journal of Pharmacology"},{"bibliographic_title":"European Journal of Pharmacology"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ejphar.2006.09.044","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"17070518","subitem_relation_type_select":"PMID"}},{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.ejphar.2006.09.044","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"EurJPharm_553_61.pdf","filesize":[{"value":"1.8 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2006849/files/EurJPharm_553_61.pdf"},"version_id":"f057b878-6551-432e-a505-72aa565af5cc"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"17066"},"item_title":"2-Aminopurine inhibits leptin receptor signal transduction","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2006849","relation_version_is_last":true,"title":["2-Aminopurine inhibits leptin receptor signal transduction"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T08:33:10.570298+00:00"}