| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
High permissivity of the fish cell line SSN-1 for piscine nodaviruses. |
|
言語 |
en |
| 作成者 |
Iwamoto, Tokinori
Mori, Koh-ichiro
Arimoto, Misao
Nakai, Toshihiro
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 1999 Inter-Research. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Nodavirus |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Viral nervous necrosis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Viral encephalopathy and retinopathy |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
SSN-1 cell line |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
FAT |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
RFLP |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
RT-PCR |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
480 |
| 内容記述 |
|
|
内容記述 |
Seventeen isolates of piscine nodavirus from larvae or juveniles of 13 marine fish species affected with viral nervous necrosis (VNN) were examined for their infectivity to a fish cell line SSN-1. Based on cytopathic effects (CPE) and virus antigen detection by fluorescent antibody technique (FAT) after incubation at 25°C, the infectivity of these virus isolates was divided into 4 groups. Group 1, including 9 virus isolates from 4 species of grouper, 2 species of sea bass, barramundi, rock porgy, and Japanese flounder showed CPE characterized by rounded, granular cells with heavy cytoplasmic vacuoles within 3 d post-incubation (p.i.), and the monolayer partially or completely disintegrated over 3 to 6 d p.i. Scattered FAT-positive cells appeared at 3 h p.i. and spread through the cell sheet with an increasing fluorescence signal over 24 h p.i. Group 2, consisting of 3 virus isolates from striped jack, induced CPE with thin or rounded, granular, refractile cells without conspicuous vacuole formation, and extensive FAT-positive reaction was observed in a time course similar to that of Group 1. Cells inoculated with Group 3 (1 isolate from tiger puffer) developed no distinct CPE but viral infection was evidenced by localized FAT-positive cells. There were no FAT-positive cells in Group 4, which included 4 isolates from Japanese flounder, Pacific cod and Atlantic halibut. However, when incubation was performed at 20°C, the SSN-1 cells inoculated with the Group 3 isolate showed CPE similar to that of Group 1 and extensive FAT-positive reaction. Evidence of virus proliferation at 20°C was also obtained in Group 4 isolates. The virus titers in the infected fish varied from 1011 to 1016 tissue culture infectious dose (TCID50) g-1 of fish. There is a good correlation between these infectivities to the SSN-1 cells and the coat protein gene genotypes of the isolates. The present results indicate that SSN-1 cells are useful for propagating and differentiating genotypic variants of piscine nodavirus. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Inter-Research |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.3354/dao039037 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.3354/dao039037 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0177-5103 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1616-1580 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10443976 |
| 開始ページ |
|
|
開始ページ |
37 |
| 書誌情報 |
Diseases of Aquatic Organisms
Diseases of Aquatic Organisms
巻 39,
号 1,
p. 37-47,
発行日 1999-12-22
|
| 旧ID |
25659 |
dao039p037.pdf (2.3 MB)
