{"created":"2025-02-21T03:31:09.160452+00:00","id":2006758,"links":{},"metadata":{"_buckets":{"deposit":"f452702d-f9df-44ed-940f-958c0c046073"},"_deposit":{"created_by":41,"id":"2006758","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006758"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006758","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"High permissivity of the fish cell line SSN-1 for piscine nodaviruses.","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Iwamoto, Tokinori","creatorNameLang":"en"}],"familyNames":[{"familyName":"Iwamoto","familyNameLang":"en"}],"givenNames":[{"givenName":"Tokinori","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mori, Koh-ichiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Mori","familyNameLang":"en"}],"givenNames":[{"givenName":"Koh-ichiro","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Arimoto, Misao","creatorNameLang":"en"}],"familyNames":[{"familyName":"Arimoto","familyNameLang":"en"}],"givenNames":[{"givenName":"Misao","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakai, Toshihiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Nakai","familyNameLang":"en"}],"givenNames":[{"givenName":"Toshihiro","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 1999 Inter-Research."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Nodavirus","subitem_subject_scheme":"Other"},{"subitem_subject":"Viral nervous necrosis","subitem_subject_scheme":"Other"},{"subitem_subject":"Viral encephalopathy and retinopathy","subitem_subject_scheme":"Other"},{"subitem_subject":"SSN-1 cell line","subitem_subject_scheme":"Other"},{"subitem_subject":"FAT","subitem_subject_scheme":"Other"},{"subitem_subject":"RFLP","subitem_subject_scheme":"Other"},{"subitem_subject":"RT-PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"480","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Seventeen isolates of piscine nodavirus from larvae or juveniles of 13 marine fish species affected with viral nervous necrosis (VNN) were examined for their infectivity to a fish cell line SSN-1. Based on cytopathic effects (CPE) and virus antigen detection by fluorescent antibody technique (FAT) after incubation at 25°C, the infectivity of these virus isolates was divided into 4 groups. Group 1, including 9 virus isolates from 4 species of grouper, 2 species of sea bass, barramundi, rock porgy, and Japanese flounder showed CPE characterized by rounded, granular cells with heavy cytoplasmic vacuoles within 3 d post-incubation (p.i.), and the monolayer partially or completely disintegrated over 3 to 6 d p.i. Scattered FAT-positive cells appeared at 3 h p.i. and spread through the cell sheet with an increasing fluorescence signal over 24 h p.i. Group 2, consisting of 3 virus isolates from striped jack, induced CPE with thin or rounded, granular, refractile cells without conspicuous vacuole formation, and extensive FAT-positive reaction was observed in a time course similar to that of Group 1. Cells inoculated with Group 3 (1 isolate from tiger puffer) developed no distinct CPE but viral infection was evidenced by localized FAT-positive cells. There were no FAT-positive cells in Group 4, which included 4 isolates from Japanese flounder, Pacific cod and Atlantic halibut. However, when incubation was performed at 20°C, the SSN-1 cells inoculated with the Group 3 isolate showed CPE similar to that of Group 1 and extensive FAT-positive reaction. Evidence of virus proliferation at 20°C was also obtained in Group 4 isolates. The virus titers in the infected fish varied from 1011 to 1016 tissue culture infectious dose (TCID50) g-1 of fish. There is a good correlation between these infectivities to the SSN-1 cells and the coat protein gene genotypes of the isolates. The present results indicate that SSN-1 cells are useful for propagating and differentiating genotypic variants of piscine nodavirus.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Inter-Research"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0177-5103","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1616-1580","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA10443976","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"37"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-12-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"47","bibliographicPageStart":"37","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"Diseases of Aquatic Organisms"},{"bibliographic_title":"Diseases of Aquatic Organisms"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3354/dao039037","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.3354/dao039037","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"dao039p037.pdf","filesize":[{"value":"2.3 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2006758/files/dao039p037.pdf"},"version_id":"4171096b-b6cf-4970-a478-cac79dd21f38"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"25659"},"item_title":"High permissivity of the fish cell line SSN-1 for piscine nodaviruses.","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2006758","relation_version_is_last":true,"title":["High permissivity of the fish cell line SSN-1 for piscine nodaviruses."],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T08:20:54.146767+00:00"}