| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Shed Blood-derived Cells from Total Hip Arthroplasty Have Osteoinductive Potential : A Pilot Study |
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言語 |
en |
| 作成者 |
Yoshida, Tomokazu
Ishikawa, Masakazu
Yasunaga, Yuji
Yamasaki, Takuma
Ochi, Mitsuo
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| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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|
権利情報 |
Copyright (c) 2010 Springer |
| 主題 |
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主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
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|
内容記述 |
Background: Cell therapy using autologous cells has been used in the treatment of various medical conditions. The mononuclear cell (MNC) fraction of bone marrow (BM) contains stem/progenitor cells that could contribute to osteogenesis and angiogenesis. Questions/purposes: We asked whether MNCs derived from intraoperative shed blood (SB), consisting of peripheral blood and BM, have osteoinductive and angiogenic potential. Methods: We harvested SB and BM from six patients undergoing THA. Isolated MNCs from SB and BM were analyzed by flow cytometry to evaluate the CD34+ cell fraction and 1 × 106 cells were seeded on an interconnective porous calcium hydroxyapatite ceramic (IP-CHA) and transplanted in the backs of athymic rats. IP-CHAs without cells were transplanted as controls and all composites were harvested after 4 and 8 weeks. Osteoinductive potential was evaluated by histologic observation, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) using anti-osteocalcin (OC) antibodies qualitatively and quantitatively. To evaluate angiogenic potential, capillary density was measured by immunohistochemistry using Isolectin B4 4 weeks after implantation. Results: We found that CD34+ cells existed in SB-MNCs and there was a trend toward lower frequency compared with BM-MNCs. Histologic osteoinduction, OC expression, and capillary density were increased by transplantation of MNCs from SB. Similar results were achieved with MNCs from BM. Conclusions: MNCs from SB have equivalent osteoinductive and angiogenic potential compared with those from BM. Clinical Relevance: SB could be an attractive source for isolation of MNCs, enhancing osteoinduction and neovascularization, to augment the reconstruction of skeletal defects. |
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言語 |
en |
| 出版者 |
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|
出版者 |
springer |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
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|
関連名称 |
The original publication is available at www.springerlink.com |
| 関連情報 |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1007/s11999-010-1444-z |
| 関連情報 |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1007/s11999-010-1444-z |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0009-921X |
| 収録物識別子 |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00607942 |
| 開始ページ |
|
|
開始ページ |
2725 |
| 書誌情報 |
Clinical Orthopaedics and Related Research
Clinical Orthopaedics and Related Research
巻 468,
号 10,
p. 2725-2733,
発行日 2010-10
|
| 旧ID |
30804 |
ClinOrthopRelatRes_468_2725.pdf (873.8 KB)
