{"created":"2025-02-21T03:28:20.445993+00:00","id":2006669,"links":{},"metadata":{"_buckets":{"deposit":"42804bf6-cc61-4881-9de2-974964ed9398"},"_deposit":{"created_by":41,"id":"2006669","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006669"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006669","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Shed Blood-derived Cells from Total Hip Arthroplasty Have Osteoinductive Potential : A Pilot Study","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yoshida, Tomokazu","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yoshida","familyNameLang":"en"}],"givenNames":[{"givenName":"Tomokazu","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ishikawa, Masakazu","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ishikawa","familyNameLang":"en"}],"givenNames":[{"givenName":"Masakazu","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yasunaga, Yuji","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yasunaga","familyNameLang":"en"}],"givenNames":[{"givenName":"Yuji","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamasaki, Takuma","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yamasaki","familyNameLang":"en"}],"givenNames":[{"givenName":"Takuma","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ochi, Mitsuo","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ochi","familyNameLang":"en"}],"givenNames":[{"givenName":"Mitsuo","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2010 Springer"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Background: Cell therapy using autologous cells has been used in the treatment of various medical conditions. The mononuclear cell (MNC) fraction of bone marrow (BM) contains stem/progenitor cells that could contribute to osteogenesis and angiogenesis.    Questions/purposes: We asked whether MNCs derived from intraoperative shed blood (SB), consisting of peripheral blood and BM, have osteoinductive and angiogenic potential.    Methods: We harvested SB and BM from six patients undergoing THA. Isolated MNCs from SB and BM were analyzed by flow cytometry to evaluate the CD34+ cell fraction and 1 × 106 cells were seeded on an interconnective porous calcium hydroxyapatite ceramic (IP-CHA) and transplanted in the backs of athymic rats. IP-CHAs without cells were transplanted as controls and all composites were harvested after 4 and 8 weeks. Osteoinductive potential was evaluated by histologic observation, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) using anti-osteocalcin (OC) antibodies qualitatively and quantitatively. To evaluate angiogenic potential, capillary density was measured by immunohistochemistry using Isolectin B4 4 weeks after implantation.    Results: We found that CD34+ cells existed in SB-MNCs and there was a trend toward lower frequency compared with BM-MNCs. Histologic osteoinduction, OC expression, and capillary density were increased by transplantation of MNCs from SB. Similar results were achieved with MNCs from BM.    Conclusions: MNCs from SB have equivalent osteoinductive and angiogenic potential compared with those from BM.    Clinical Relevance: SB could be an attractive source for isolation of MNCs, enhancing osteoinduction and neovascularization, to augment the reconstruction of skeletal defects.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"springer"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0009-921X","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00607942","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"2725"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"2733","bibliographicPageStart":"2725","bibliographicVolumeNumber":"468","bibliographic_titles":[{"bibliographic_title":"Clinical Orthopaedics and Related Research"},{"bibliographic_title":"Clinical Orthopaedics and Related Research"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"The original publication is available at www.springerlink.com"}]},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s11999-010-1444-z","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1007/s11999-010-1444-z","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"ClinOrthopRelatRes_468_2725.pdf","filesize":[{"value":"873.8 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2006669/files/ClinOrthopRelatRes_468_2725.pdf"},"version_id":"9762896e-23ce-4f22-be0f-84a0208b0911"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"30804"},"item_title":"Shed Blood-derived Cells from Total Hip Arthroplasty Have Osteoinductive Potential : A Pilot Study","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2006669","relation_version_is_last":true,"title":["Shed Blood-derived Cells from Total Hip Arthroplasty Have Osteoinductive Potential : A Pilot Study"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T08:07:38.394956+00:00"}