| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Characterization of CO3Ap-collagen sponges using X-ray high-resolution microtomography |
|
言語 |
en |
| 作成者 |
Itoh, Manabu
Shimazu, Atsushi
Hirata, Isao
Yoshida, Yasuhiro
Shintani, Hideaki
Okazaki, Masayuki
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2003 Elsevier Ltd. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Bone repair |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Collagen |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Hydroxyapatite composite |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Scaffold |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
For reconstruction and regeneration of hard tissues, scaffold biomaterials with large size pores and high porosity are important, in addition to their roles as supporting frames. To develop a new biodegradable scaffold biomaterial, CO3Ap, which has crystallinity and a chemical composition similar to bone, was synthesized at pH 7.4 and 60°C. Then, the CO3Ap was mixed with a neutralized collagen gel and the CO3Ap-collagen mixtures with different kinds of CO3Ap contents and porosity were lyophilized into sponges. Scanning electron micrography (SEM) observation of CO3Ap-collagen sponges showed favorable pores for cell invasion. Approximately 50–300 μm size pores appeared to continue through the bulk. Higher magnification of the sponge showed a better adhesion between CO3Ap crystals and collagen. X-ray high-resolution microtomography revealed a clear image of the 3D structure of the sponges. The porosity of 0, 70 and 90%(w/w) CO3Ap-collagen sponges was 79.2±2.8%, 72.6±2.4% and 48.9±6.1%, respectively. The 70%(w/w) CO3Ap-collagen sponge appeared to be the most favorable biomaterial from the viewpoint of natural bone properties. Mouse osteoblast MC3T3-E1 cells were cultured in αMEM with 10% FCS for 2 weeks. Hematoxylin–eosin staining confirmed osteoblast cells invaded well into the CO3Ap-collagen sponge. These sponges are expected to be used as hard tissue scaffold biomaterials for therapeutic uses. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.biomaterials.2003.09.071 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.biomaterials.2003.09.071 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0142-9612 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00110092 |
| 開始ページ |
|
|
開始ページ |
2577 |
| 書誌情報 |
Biomaterials
Biomaterials
巻 25,
号 13,
p. 2577-2583,
発行日 2004-06
|
| 旧ID |
21545 |
Biomaterials_25_2577.pdf (1.5 MB)
