| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Participation of thioredoxin in the V(V)-reduction reaction by Vanabin2 |
|
言語 |
en |
| 作成者 |
Ueki, Tatsuya
Uwagaki, Masayuki
Yamamoto, Sohei
Michibata, Hitoshi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2014 Elsevier B.V. All rights reserved. |
| 権利情報 |
|
|
権利情報 |
This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Ascidian |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Vanadium |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Reductase |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Metal |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Redox |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
Background: It is well-understood that ascidians accumulate high levels of vanadium, a reduced form of V(III), in an extremely acidic vacuole in their blood cells. Vanabins are small cysteine-rich proteins that have been identified only from vanadium-rich ascidians. A previous study revealed that Vanabin2 can act as a V(V)-reductase in the glutathione cascade. Methods: AsTrx1, A thioredoxin gene, was cloned from the vanadium-rich ascidian, Ascidia sydneiensis samea, by PCR. AsTrx1 and Vanabin2 were prepared as recombinant proteins, and V(V)-reduction by Vanabin2 was assessed by ESR and ion-exchange column chromatography. Site-directed mutagenesis was performed to examine the direct involvement of cysteine residues. Tissue expression of AsTrx1 was also examined by RT-PCR. Results: When reduced AsTrx1 and Vanabin2 were combined, Vanabin2 adopted an SS/SH intermediate structure while V(V) was reduced to V(IV). The loss of cysteine residues in either Vanabin2 or AsTrx1 caused a significant loss of reductase activity. Vapp and Kapp values for Vanabin2-catalyzed V(V)-reduction in the thioredoxin cascade were 0.066 mol-V(IV)/min/mol-Vanabin2 and 0.19 mM, respectively. The Kapp value was 2.7-fold lower than that observed in the glutathione cascade. The AsTrx1 gene was expressed at a very high level in blood cells, in which Vanabins 1–4 were co-expressed. Conclusions: AsTrx1 may contribute to a significant part of the redox cascade for V(V)-reduction by Vanabin2 in the cytoplasm of vanadocytes, but prevails only at low V(V) concentrations. General significance: This study is the first to report the reduction of V(V) in the thioredoxin cascade. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This work was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan (Nos. 20570070, 21570077, 22224011, 25120508 and 25440170). |
| 出版者 |
|
|
出版者 |
Elsevier B.V. |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.bbagen.2014.07.023 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://doi.org/10.1016/j.bbagen.2014.07.023 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0304-4165 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00564679 |
| 開始ページ |
|
|
開始ページ |
3238 |
| 書誌情報 |
Biochimica et Biophysica Acta (BBA) - General Subjects
Biochimica et Biophysica Acta (BBA) - General Subjects
巻 1840,
号 11,
p. 3238-3245,
発行日 2014-11
|
| 旧ID |
39966 |
BBAGenSub_1840_3238.pdf (1.1 MB)
