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Participation of thioredoxin in the V(V)-reduction reaction by Vanabin2

https://hiroshima.repo.nii.ac.jp/records/2006390
https://hiroshima.repo.nii.ac.jp/records/2006390
a2dbb6c0-1903-4097-b718-edcb2a1a7613
名前 / ファイル ライセンス アクション
BBAGenSub_1840_3238.pdf BBAGenSub_1840_3238.pdf (1.1 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Participation of thioredoxin in the V(V)-reduction reaction by Vanabin2
言語 en
作成者 Ueki, Tatsuya

× Ueki, Tatsuya

en Ueki, Tatsuya

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Uwagaki, Masayuki

× Uwagaki, Masayuki

en Uwagaki, Masayuki

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Yamamoto, Sohei

× Yamamoto, Sohei

en Yamamoto, Sohei

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Michibata, Hitoshi

× Michibata, Hitoshi

en Michibata, Hitoshi

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 Copyright (c) 2014 Elsevier B.V. All rights reserved.
権利情報
権利情報 This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
主題
主題Scheme Other
主題 Ascidian
主題
主題Scheme Other
主題 Vanadium
主題
主題Scheme Other
主題 Reductase
主題
主題Scheme Other
主題 Metal
主題
主題Scheme Other
主題 Redox
主題
主題Scheme NDC
主題 460
内容記述
内容記述 Background: It is well-understood that ascidians accumulate high levels of vanadium, a reduced form of V(III), in an extremely acidic vacuole in their blood cells. Vanabins are small cysteine-rich proteins that have been identified only from vanadium-rich ascidians. A previous study revealed that Vanabin2 can act as a V(V)-reductase in the glutathione cascade. Methods: AsTrx1, A thioredoxin gene, was cloned from the vanadium-rich ascidian, Ascidia sydneiensis samea, by PCR. AsTrx1 and Vanabin2 were prepared as recombinant proteins, and V(V)-reduction by Vanabin2 was assessed by ESR and ion-exchange column chromatography. Site-directed mutagenesis was performed to examine the direct involvement of cysteine residues. Tissue expression of AsTrx1 was also examined by RT-PCR. Results: When reduced AsTrx1 and Vanabin2 were combined, Vanabin2 adopted an SS/SH intermediate structure while V(V) was reduced to V(IV). The loss of cysteine residues in either Vanabin2 or AsTrx1 caused a significant loss of reductase activity. Vapp and Kapp values for Vanabin2-catalyzed V(V)-reduction in the thioredoxin cascade were 0.066 mol-V(IV)/min/mol-Vanabin2 and 0.19 mM, respectively. The Kapp value was 2.7-fold lower than that observed in the glutathione cascade. The AsTrx1 gene was expressed at a very high level in blood cells, in which Vanabins 1–4 were co-expressed. Conclusions: AsTrx1 may contribute to a significant part of the redox cascade for V(V)-reduction by Vanabin2 in the cytoplasm of vanadocytes, but prevails only at low V(V) concentrations. General significance: This study is the first to report the reduction of V(V) in the thioredoxin cascade.
言語 en
内容記述
内容記述タイプ Other
内容記述 This work was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan (Nos. 20570070, 21570077, 22224011, 25120508 and 25440170).
出版者
出版者 Elsevier B.V.
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ AO
出版タイプResource http://purl.org/coar/version/c_b1a7d7d4d402bcce
関連情報
識別子タイプ DOI
関連識別子 10.1016/j.bbagen.2014.07.023
関連情報
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 http://doi.org/10.1016/j.bbagen.2014.07.023
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0304-4165
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AA00564679
開始ページ
開始ページ 3238
書誌情報 Biochimica et Biophysica Acta (BBA) - General Subjects
Biochimica et Biophysica Acta (BBA) - General Subjects

巻 1840, 号 11, p. 3238-3245, 発行日 2014-11
旧ID 39966
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