{"created":"2025-02-21T03:19:56.285689+00:00","id":2006390,"links":{},"metadata":{"_buckets":{"deposit":"b70786b2-08cf-4bfd-9662-03f2279020e0"},"_deposit":{"created_by":41,"id":"2006390","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006390"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006390","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Participation of thioredoxin in the V(V)-reduction reaction by Vanabin2","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ueki, Tatsuya","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ueki","familyNameLang":"en"}],"givenNames":[{"givenName":"Tatsuya","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Uwagaki, Masayuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Uwagaki","familyNameLang":"en"}],"givenNames":[{"givenName":"Masayuki","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamamoto, Sohei","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yamamoto","familyNameLang":"en"}],"givenNames":[{"givenName":"Sohei","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Michibata, Hitoshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Michibata","familyNameLang":"en"}],"givenNames":[{"givenName":"Hitoshi","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2014 Elsevier B.V. All rights reserved."},{"subitem_rights":"This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":" Ascidian","subitem_subject_scheme":"Other"},{"subitem_subject":"Vanadium","subitem_subject_scheme":"Other"},{"subitem_subject":"Reductase","subitem_subject_scheme":"Other"},{"subitem_subject":"Metal","subitem_subject_scheme":"Other"},{"subitem_subject":"Redox","subitem_subject_scheme":"Other"},{"subitem_subject":"460","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Background: It is well-understood that ascidians accumulate high levels of vanadium, a reduced form of V(III), in an extremely acidic vacuole in their blood cells. Vanabins are small cysteine-rich proteins that have been identified only from vanadium-rich ascidians. A previous study revealed that Vanabin2 can act as a V(V)-reductase in the glutathione cascade.     Methods: AsTrx1, A thioredoxin gene, was cloned from the vanadium-rich ascidian, Ascidia sydneiensis samea, by PCR. AsTrx1 and Vanabin2 were prepared as recombinant proteins, and V(V)-reduction by Vanabin2 was assessed by ESR and ion-exchange column chromatography. Site-directed mutagenesis was performed to examine the direct involvement of cysteine residues. Tissue expression of AsTrx1 was also examined by RT-PCR.     Results: When reduced AsTrx1 and Vanabin2 were combined, Vanabin2 adopted an SS/SH intermediate structure while V(V) was reduced to V(IV). The loss of cysteine residues in either Vanabin2 or AsTrx1 caused a significant loss of reductase activity. Vapp and Kapp values for Vanabin2-catalyzed V(V)-reduction in the thioredoxin cascade were 0.066 mol-V(IV)/min/mol-Vanabin2 and 0.19 mM, respectively. The Kapp value was 2.7-fold lower than that observed in the glutathione cascade. The AsTrx1 gene was expressed at a very high level in blood cells, in which Vanabins 1–4 were co-expressed.     Conclusions: AsTrx1 may contribute to a significant part of the redox cascade for V(V)-reduction by Vanabin2 in the cytoplasm of vanadocytes, but prevails only at low V(V) concentrations.     General significance: This study is the first to report the reduction of V(V) in the thioredoxin cascade.","subitem_description_language":"en"},{"subitem_description":"This work was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan (Nos. 20570070, 21570077, 22224011, 25120508 and 25440170).","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0304-4165","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00564679","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"3238"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"3245","bibliographicPageStart":"3238","bibliographicVolumeNumber":"1840","bibliographic_titles":[{"bibliographic_title":"Biochimica et Biophysica Acta (BBA) - General Subjects"},{"bibliographic_title":"Biochimica et Biophysica Acta (BBA) - General Subjects"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbagen.2014.07.023","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://doi.org/10.1016/j.bbagen.2014.07.023","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"BBAGenSub_1840_3238.pdf","filesize":[{"value":"1.1 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2006390/files/BBAGenSub_1840_3238.pdf"},"version_id":"f5a47ae1-06d5-4eea-b710-5cba58062e00"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"39966"},"item_title":"Participation of thioredoxin in the V(V)-reduction reaction by Vanabin2","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2006390","relation_version_is_last":true,"title":["Participation of thioredoxin in the V(V)-reduction reaction by Vanabin2"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T07:29:12.043320+00:00"}