| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
The extract of syngeneic keratinocytes enhances IgE production from BALB/c mouse splenic lymphocytes in vitro. |
|
言語 |
en |
| 作成者 |
Morimoto, Kenichi
Nabeshima, Yukiko
Mochizuki, Mitsuru
Oomizu, Souichi
Yamamoto, Shoso
Hide, Michihiro
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2006 Springer-Verlag. "The original publication is available at www.springerlink.com" |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
immunoglobulin E |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
keratinocyte |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
B-lymphocyte |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
immunoglobulin class switching |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
atopic dermatitis |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
Background: The increase of serum IgE levels is closely associated with atopic dermatitis. We have previously revealed that cellular extract of PAM212 cells (PAM-extract), BALB/c mouse keratinocyte cell line, induced a remarkable increase of serum IgE levels, in vivo, when subcutaneously injected into BALB/c mice. However, precise mechanism of IgE-increasing activity was unclear. Objective: To elucidate the mechanism of IgE-increase in sera of BALB/c mice induced by PAM-extract, we explored the direct influence of PAM-extract on immunoglobulin production and class-switching in the culture of splenic lymphocytes and purified B-cells, in vitro. Methods: Splenic lymphocytes or purified B-cells obtained from BALB/c mice were cultured with various combinations of IL-4, anti-CD40 antibody, and PAM-extract for seven days. IgE and IgG concentrations of culture supernatants were measured by ELISA. Epsilon germ-line transcriptions were assessed by RT-PCR from the cultured cells. Results: IgE and IgG concentrations in culture supernatant of splenic lymphocytes were increased by an addition of PAM-extract in the presence of both IL-4 and anti-CD40 antibody. Epsilon germ-line transcript was also induced in parallel to the increase of IgE production. Similar results were obtained when purified B-cells were employed in stead of whole splenic lymphocytes. Conclusion: The cellular extract of keratinocyte promotes immunoglobulin class-switching to IgE and IgE production from mouse splenic B-cells in an IL-4- and CD40-stimuli-dependent manner. Such enhancement may account for the increase of serum IgE in patients with dermatitis in association with a Th2 microenvironment. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Springer |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1007/s00403-005-0636-y |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
16402182 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1007/s00403-005-0636-y |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0340-3696 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA0054724X |
| 開始ページ |
|
|
開始ページ |
358 |
| 書誌情報 |
Archives of Dermatological Research
Archives of Dermatological Research
巻 297,
号 8,
p. 358-366,
発行日 2006-02
|
| 旧ID |
17038 |
ArchDermatolRes_297_358.pdf (1.3 MB)
