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The extract of syngeneic keratinocytes enhances IgE production from BALB/c mouse splenic lymphocytes in vitro.

https://hiroshima.repo.nii.ac.jp/records/2006304
https://hiroshima.repo.nii.ac.jp/records/2006304
3ecba464-b9d7-4a3e-8b3a-d6b69cc3c6e1
名前 / ファイル ライセンス アクション
ArchDermatolRes_297_358.pdf ArchDermatolRes_297_358.pdf (1.3 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル The extract of syngeneic keratinocytes enhances IgE production from BALB/c mouse splenic lymphocytes in vitro.
言語 en
作成者 Morimoto, Kenichi

× Morimoto, Kenichi

en Morimoto, Kenichi

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Nabeshima, Yukiko

× Nabeshima, Yukiko

en Nabeshima, Yukiko

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Mochizuki, Mitsuru

× Mochizuki, Mitsuru

en Mochizuki, Mitsuru

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Oomizu, Souichi

× Oomizu, Souichi

en Oomizu, Souichi

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Yamamoto, Shoso

× Yamamoto, Shoso

en Yamamoto, Shoso

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Hide, Michihiro

× Hide, Michihiro

en Hide, Michihiro

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 Copyright (c) 2006 Springer-Verlag. "The original publication is available at www.springerlink.com"
主題
主題Scheme Other
主題 immunoglobulin E
主題
主題Scheme Other
主題 keratinocyte
主題
主題Scheme Other
主題 B-lymphocyte
主題
主題Scheme Other
主題 immunoglobulin class switching
主題
主題Scheme Other
主題 atopic dermatitis
主題
主題Scheme NDC
主題 490
内容記述
内容記述 Background: The increase of serum IgE levels is closely associated with atopic dermatitis. We have previously revealed that cellular extract of PAM212 cells (PAM-extract), BALB/c mouse keratinocyte cell line, induced a remarkable increase of serum IgE levels, in vivo, when subcutaneously injected into BALB/c mice. However, precise mechanism of IgE-increasing activity was unclear. Objective: To elucidate the mechanism of IgE-increase in sera of BALB/c mice induced by PAM-extract, we explored the direct influence of PAM-extract on immunoglobulin production and class-switching in the culture of splenic lymphocytes and purified B-cells, in vitro. Methods: Splenic lymphocytes or purified B-cells obtained from BALB/c mice were cultured with various combinations of IL-4, anti-CD40 antibody, and PAM-extract for seven days. IgE and IgG concentrations of culture supernatants were measured by ELISA. Epsilon germ-line transcriptions were assessed by RT-PCR from the cultured cells. Results: IgE and IgG concentrations in culture supernatant of splenic lymphocytes were increased by an addition of PAM-extract in the presence of both IL-4 and anti-CD40 antibody. Epsilon germ-line transcript was also induced in parallel to the increase of IgE production. Similar results were obtained when purified B-cells were employed in stead of whole splenic lymphocytes. Conclusion: The cellular extract of keratinocyte promotes immunoglobulin class-switching to IgE and IgE production from mouse splenic B-cells in an IL-4- and CD40-stimuli-dependent manner. Such enhancement may account for the increase of serum IgE in patients with dermatitis in association with a Th2 microenvironment.
言語 en
出版者
出版者 Springer
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ AO
出版タイプResource http://purl.org/coar/version/c_b1a7d7d4d402bcce
関連情報
識別子タイプ DOI
関連識別子 10.1007/s00403-005-0636-y
関連情報
識別子タイプ PMID
関連識別子 16402182
関連情報
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 http://dx.doi.org/10.1007/s00403-005-0636-y
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0340-3696
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AA0054724X
開始ページ
開始ページ 358
書誌情報 Archives of Dermatological Research
Archives of Dermatological Research

巻 297, 号 8, p. 358-366, 発行日 2006-02
旧ID 17038
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