{"created":"2025-02-21T03:17:10.383249+00:00","id":2006304,"links":{},"metadata":{"_buckets":{"deposit":"2e33b805-7e79-4644-812d-631f2c17306a"},"_deposit":{"created_by":41,"id":"2006304","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006304"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006304","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"The extract of syngeneic keratinocytes enhances IgE production from BALB/c mouse splenic lymphocytes in vitro.","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Morimoto, Kenichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Morimoto","familyNameLang":"en"}],"givenNames":[{"givenName":"Kenichi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nabeshima, Yukiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Nabeshima","familyNameLang":"en"}],"givenNames":[{"givenName":"Yukiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mochizuki, Mitsuru","creatorNameLang":"en"}],"familyNames":[{"familyName":"Mochizuki","familyNameLang":"en"}],"givenNames":[{"givenName":"Mitsuru","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Oomizu, Souichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Oomizu","familyNameLang":"en"}],"givenNames":[{"givenName":"Souichi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamamoto, Shoso","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yamamoto","familyNameLang":"en"}],"givenNames":[{"givenName":"Shoso","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hide, Michihiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Hide","familyNameLang":"en"}],"givenNames":[{"givenName":"Michihiro","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2006 Springer-Verlag. \"The original publication is available at www.springerlink.com\""}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"immunoglobulin E","subitem_subject_scheme":"Other"},{"subitem_subject":"keratinocyte","subitem_subject_scheme":"Other"},{"subitem_subject":"B-lymphocyte","subitem_subject_scheme":"Other"},{"subitem_subject":"immunoglobulin class switching","subitem_subject_scheme":"Other"},{"subitem_subject":"atopic dermatitis","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Background: The increase of serum IgE levels is closely associated with atopic dermatitis. We have previously revealed that cellular extract of PAM212 cells (PAM-extract), BALB/c mouse keratinocyte cell line, induced a remarkable increase of serum IgE levels, in vivo, when subcutaneously injected into BALB/c mice. However, precise mechanism of IgE-increasing activity was unclear. Objective: To elucidate the mechanism of IgE-increase in sera of BALB/c mice induced by PAM-extract, we explored the direct influence of PAM-extract on immunoglobulin production and class-switching in the culture of splenic lymphocytes and purified B-cells, in vitro. Methods: Splenic lymphocytes or purified B-cells obtained from BALB/c mice were cultured with various combinations of IL-4, anti-CD40 antibody, and PAM-extract for seven days. IgE and IgG concentrations of culture supernatants were measured by ELISA. Epsilon germ-line transcriptions were assessed by RT-PCR from the cultured cells. Results: IgE and IgG concentrations in culture supernatant of splenic lymphocytes were increased by an addition of PAM-extract in the presence of both IL-4 and anti-CD40 antibody. Epsilon germ-line transcript was also induced in parallel to the increase of IgE production. Similar results were obtained when purified B-cells were employed in stead of whole splenic lymphocytes. Conclusion: The cellular extract of keratinocyte promotes immunoglobulin class-switching to IgE and IgE production from mouse splenic B-cells in an IL-4- and CD40-stimuli-dependent manner. Such enhancement may account for the increase of serum IgE in patients with dermatitis in association with a Th2 microenvironment.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Springer"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0340-3696","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA0054724X","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"358"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"366","bibliographicPageStart":"358","bibliographicVolumeNumber":"297","bibliographic_titles":[{"bibliographic_title":"Archives of Dermatological Research"},{"bibliographic_title":"Archives of Dermatological Research"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s00403-005-0636-y","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"16402182","subitem_relation_type_select":"PMID"}},{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1007/s00403-005-0636-y","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"ArchDermatolRes_297_358.pdf","filesize":[{"value":"1.3 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2006304/files/ArchDermatolRes_297_358.pdf"},"version_id":"88da4253-aa97-4fc4-905d-013589255100"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"17038"},"item_title":"The extract of syngeneic keratinocytes enhances IgE production from BALB/c mouse splenic lymphocytes in vitro.","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2006304","relation_version_is_last":true,"title":["The extract of syngeneic keratinocytes enhances IgE production from BALB/c mouse splenic lymphocytes in vitro."],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T07:17:38.992080+00:00"}