| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Construction of a simple biocatalyst using psychrophilic bacterial cells and its application for efficient 3-hydroxypropionaldehyde production from glycerol |
|
言語 |
en |
| 作成者 |
Tajima, Takahisa
Fuki, Koji
Kataoka, Naoya
Kudou, Daizou
Nakashimada, Yutaka
Kato, Junichi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
© Tajima et al.; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Biocatalysis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Psychrophilic bacterium |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
3-hydroxypropionaldehyde |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Biomass |
| 内容記述 |
|
|
内容記述 |
Most whole cell biocatalysts have some problems with yields and productivities because of various metabolites produced as byproducts and limitations of substrate uptake. We propose a psychrophile-based simple biocatalyst for efficient bio-production using mesophilic enzymes expressed in psychrophilic Shewanella livingstonensis Ac10 cells whose basic metabolism was inactivated by heat treatment. The 45°C heat-treated cells expressing lacZ showed maximum beta-galactosidase activity as well as chloroform/SDS-treated cells to increase membrane permeability. The fluorescent dye 5-cyano-2,3-ditolyl-tetrazolium chloride staining indicated that most basic metabolism of Ac10 was lost by heat treatment at 45˚C for 10 min. The simple biocatalyst was applied for 3-HPA production by using Klebsiella pneumoniae dhaB genes. 3-HPA was stoichiometrically produced with the complete consumption of glycerol at a high production rate of 8.85 mmol 3-HPA/g dry cell/h. The amount of 3-HPA production increased by increasing the concentrations of biocatalyst and glycerol. Furthermore, it could convert biodiesel-derived crude glycerol to 3-HPA. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
We thank Professor Tatsuo Kurihara in Kyoto University for providing S. livingstonenesis Ac10-Rifr. |
| 出版者 |
|
|
出版者 |
Springer Nature |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1186/2191-0855-3-69 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1186/2191-0855-3-69 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
24314120 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2191-0855 |
| 開始ページ |
|
|
開始ページ |
69 |
| 書誌情報 |
AMB Express
AMB Express
巻 3,
号 1,
p. 69,
発行日 2013-12-05
|
| 旧ID |
46766 |
2191-0855-3-69.pdf (352.4 KB)
