{"created":"2025-02-21T03:13:21.753666+00:00","id":2006185,"links":{},"metadata":{"_buckets":{"deposit":"8ad89f52-bf7e-4631-9c43-11647635ca78"},"_deposit":{"created_by":41,"id":"2006185","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006185"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006185","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Construction of a simple biocatalyst using psychrophilic bacterial cells and its application for efficient 3-hydroxypropionaldehyde production from glycerol","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tajima, Takahisa","creatorNameLang":"en"}],"familyNames":[{"familyName":"Tajima","familyNameLang":"en"}],"givenNames":[{"givenName":"Takahisa","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Fuki, Koji","creatorNameLang":"en"}],"familyNames":[{"familyName":"Fuki","familyNameLang":"en"}],"givenNames":[{"givenName":"Koji","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kataoka, Naoya","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kataoka","familyNameLang":"en"}],"givenNames":[{"givenName":"Naoya","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kudou, Daizou","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kudou","familyNameLang":"en"}],"givenNames":[{"givenName":"Daizou","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakashimada, Yutaka ","creatorNameLang":"en"}],"familyNames":[{"familyName":"Nakashimada","familyNameLang":"en"}],"givenNames":[{"givenName":"Yutaka ","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kato, Junichi ","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kato","familyNameLang":"en"}],"givenNames":[{"givenName":"Junichi ","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© Tajima et al.; licensee Springer. 2013  This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Biocatalysis","subitem_subject_scheme":"Other"},{"subitem_subject":"Psychrophilic bacterium","subitem_subject_scheme":"Other"},{"subitem_subject":"3-hydroxypropionaldehyde","subitem_subject_scheme":"Other"},{"subitem_subject":"Biomass","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Most whole cell biocatalysts have some problems with yields and productivities because of various metabolites produced as byproducts and limitations of substrate uptake. We propose a psychrophile-based simple biocatalyst for efficient bio-production using mesophilic enzymes expressed in psychrophilic Shewanella livingstonensis Ac10 cells whose basic metabolism was inactivated by heat treatment. The 45°C heat-treated cells expressing lacZ showed maximum beta-galactosidase activity as well as chloroform/SDS-treated cells to increase membrane permeability. The fluorescent dye 5-cyano-2,3-ditolyl-tetrazolium chloride staining indicated that most basic metabolism of Ac10 was lost by heat treatment at 45˚C for 10 min. The simple biocatalyst was applied for 3-HPA production by using Klebsiella pneumoniae dhaB genes. 3-HPA was stoichiometrically produced with the complete consumption of glycerol at a high production rate of 8.85 mmol 3-HPA/g dry cell/h. The amount of 3-HPA production increased by increasing the concentrations of biocatalyst and glycerol. Furthermore, it could convert biodiesel-derived crude glycerol to 3-HPA.","subitem_description_language":"en"},{"subitem_description":"We thank Professor Tatsuo Kurihara in Kyoto University for providing S. livingstonenesis Ac10-Rifr.","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"2191-0855","subitem_source_identifier_type":"ISSN"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"69"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-12-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"69","bibliographicVolumeNumber":"3","bibliographic_titles":[{"bibliographic_title":"AMB Express"},{"bibliographic_title":"AMB Express"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1186/2191-0855-3-69","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/2191-0855-3-69","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"24314120","subitem_relation_type_select":"PMID"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"2191-0855-3-69.pdf","filesize":[{"value":"352.4 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2006185/files/2191-0855-3-69.pdf"},"version_id":"fdb987e6-95ce-4c99-a801-f8fca0ed95db"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"46766"},"item_title":"Construction of a simple biocatalyst using psychrophilic bacterial cells and its application for efficient 3-hydroxypropionaldehyde production from glycerol","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2006185","relation_version_is_last":true,"title":["Construction of a simple biocatalyst using psychrophilic bacterial cells and its application for efficient 3-hydroxypropionaldehyde production from glycerol"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T06:54:40.861261+00:00"}