| Item type |
デフォルト(1) |
| 公開日 |
2025-07-16 |
| タイトル |
|
|
タイトル |
One-pot chemo-enzymatic synthesis and one-step recovery of length-variable long-chain polyphosphates from microalgal biomass |
|
言語 |
en |
| 作成者 |
Lin, Yi-Hsuan
Nishikawa, Shota
Jia, Tony Z.
Yeh, Fang-I.
Khusnutdinova, Anna
Yakunin, Alexander F.
Fujishima, Kosuke
Wang, Po-Hsiang
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is the Accepted Manuscript of an article published in Green Chemistry. The final published version is available from the Royal Society of Chemistry at: https://doi.org/10.1039/D3GC03162D © The author(s). This manuscript is made available under the terms of the Royal Society of Chemistry's Licence to Publish. The copyright remains with the author(s). |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is not the published version. Please cite only the published version. |
| 権利情報 |
|
|
言語 |
ja |
|
権利情報 |
この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Polyphosphate |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
microalgae |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
polyphosphate kinase |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
one-pot enzyme cascade |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
bioeconomy |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
biomass valorization |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Phosphate, an essential ingredient in fertilizers and detergents used daily worldwide, is a finite resource that may be exhausted within 70 years, while improper phosphate waste disposal into aquatic environments will result in eutrophication. Despite some chemical-based methods, biological phosphorus removal using polyphosphate-accumulating organisms, such as microalgae, is a sustainable alternative to reclaim phosphate from wastewater before wastewater enters aquatic environments, preventing ecosystem damage while recovering the phosphate for industrial use. Moreover, polyphosphates have profound biological functions and biomedical applications, serving as an energy stock, drug delivery vesicles, coagulation factors, and antiviral agents depending on the length of the polyphosphate chain, showing inherent value in polyphosphate recovery. However, before this study, there were no sustainable and efficient approaches to synthesizing purified polyphosphates enriched with different lengths, which limited industrial and biomedical applications. Here, by leveraging the power of thermodynamic coupling and phase transitions, we established a one-pot, two-step multi-enzyme cascade (comprising creatine kinase and two polyphosphate kinases) to transform heterogeneous polyphosphate in microalgae biomass to insoluble long-chain polyphosphate 1300-mers, allowing for further purification in a single step. In the cascade reactions, introducing creatine as the high-energy P-shuttle enables controlled manipulation of the creatine kinase reaction direction via pH modulation, effectively circumventing the competition between the two polyphosphate kinase-mediated reactions. Finally, we optimized a thermo-digestion approach to transform polyphosphate 1300-mers into shorter polyphosphates enriched with a specific narrow length range. Therefore, the processes established here create a sustainable P bioeconomy platform to refine microalgal biomass for biotechnological use. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
The authors acknowledge Toshikazu Shiba and other employees of RegeneTiss for providing EX-polyP as standard samples, as well as performing HPLC gel-filtration analysis of the synthesized polyP 1300-mer. P.-H. W. is supported by the National Science and Technology Council of Taiwan (112-2628-E008-005). T. Z. J. is supported by the Japan Society for the Promotion of Science (JSPS) (Grant-in-aid 21K14746) and the Mizuho Foundation for the Promotion of Science. K. F. is supported by an ELSI research grant and NINS Astrobiology Center grant (AB301003 and AB311001). |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
The Royal Society of Chemistry |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 助成情報 |
|
|
|
助成機関識別子タイプ |
Crossref Funder |
|
|
助成機関識別子タイプURI |
https://doi.org/10.13039/501100001691 |
|
|
助成機関名 |
日本学術振興会 |
|
|
言語 |
ja |
|
|
助成機関名 |
Japan Society for the Promotion of Science |
|
|
言語 |
en |
|
|
研究課題番号URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-21K14746/ |
|
|
研究課題番号 |
21K14746 |
|
|
研究課題名 |
Biomolecule compartmentalization by phase separated protocells |
|
|
言語 |
en |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1463-9262 |
| 書誌情報 |
en : Green Chemistry
号 23-25,
p. 9896-9907,
発行日 2023-10-27
|
GC_23-25_9896.docx (4.7 MB)
