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  1. 広島大学の刊行物
  2. Hiroshima Journal of Medical Sciences
  3. 58巻4号

Blood Transport Method for Chromosome Analysis of Residents Living Near Semipalatinsk Nuclear Test Site

https://hiroshima.repo.nii.ac.jp/records/2013619
https://hiroshima.repo.nii.ac.jp/records/2013619
9f2c4e3c-3cb6-4b1a-bdd6-4fba96154d71
名前 / ファイル ライセンス アクション
HiroshimaJMedSci_58_67.pdf HiroshimaJMedSci_58_67.pdf (1.6 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Blood Transport Method for Chromosome Analysis of Residents Living Near Semipalatinsk Nuclear Test Site
言語 en
作成者 Rodzi, Mohd

× Rodzi, Mohd

en Rodzi, Mohd

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Ihda, Shozo

× Ihda, Shozo

en Ihda, Shozo

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Yokozeki, Masako

× Yokozeki, Masako

en Yokozeki, Masako

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Takeichi, Nobuo

× Takeichi, Nobuo

en Takeichi, Nobuo

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Tanaka, Kimio

× Tanaka, Kimio

en Tanaka, Kimio

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Hoshi, Masaharu

× Hoshi, Masaharu

en Hoshi, Masaharu

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 (c) Hiroshima University Medical Press.
主題
主題Scheme Other
主題 Whole blood
主題
主題Scheme Other
主題 Mitotic index
主題
主題Scheme Other
主題 Chromosome aberrations
主題
主題Scheme Other
主題 Nuclear explosion test
主題
主題Scheme Other
主題 Biodosimetry
主題
主題Scheme NDC
主題 490
内容記述
内容記述 A study was conducted to compare the storage conditions and transportation period for blood samples collected from residents living in areas near the Semipalatinsk nuclear test site (SNTS). Experiments were performed to simulate storage and shipping environments. Phytohaemagglutinin (PHA)-stimulated blood was stored in 15-ml tubes (condition A: current transport method) in the absence or in 50-ml flasks (condition B: previous transport method) in the presence of RPMI-1640 and 20% fetal bovine serum (FBS). Samples were kept refrigerated at 4℃ and cell viability was assessed after 3, 8, 12 and 14 days of storage. RPMI-1640, 20% FBS and further PHA were added to blood samples under condition A in 50-ml flasks for culture. Whole-blood samples under condition B were directly incubated without further sub-culturing process, neither media nor PHA were added, to adopt a similar protocol to that employed in the previous transport method. Samples in condition A and condition B were incubated for 48 hr at 37℃ and their mitotic index was determined. The results showed that viable lymphocytes were consistent in both storage conditions but the mitotic index was higher in condition A than in condition B. Although further confirmation studies have to be carried out, previous chromosomal studies and the present experiment have shown that PHA-stimulated blood could be stored without culture medium for up to 8 days under condition A. The present results will be useful for cytogenetic analysis of blood samples that have been transported long distances wherever a radiation accident has occurred.
言語 en
出版者
出版者 Hiroshima University Medical Press
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ departmental bulletin paper
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0018-2052
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AA00664312
開始ページ
開始ページ 67
書誌情報 Hiroshima Journal of Medical Sciences
Hiroshima Journal of Medical Sciences

巻 58, 号 4, p. 67-73, 発行日 2009-12
旧ID 34969
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