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  1. 広島大学の刊行物
  2. Hiroshima Journal of Medical Sciences
  3. 54巻1号

Attenuation of Telomerase Activity by Hammerhead Ribozymes Targeting Human Telomerase RNA and Telomerase Reverse Transcriptase in Pancreatic Carcinoma Cells

https://hiroshima.repo.nii.ac.jp/records/2013553
https://hiroshima.repo.nii.ac.jp/records/2013553
054c74c3-6c07-45bb-a47f-dc8018c378c6
名前 / ファイル ライセンス アクション
KJ00004161811.pdf KJ00004161811.pdf (936.2 KB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Attenuation of Telomerase Activity by Hammerhead Ribozymes Targeting Human Telomerase RNA and Telomerase Reverse Transcriptase in Pancreatic Carcinoma Cells
言語 en
作成者 Hayashidani, Yasuo

× Hayashidani, Yasuo

en Hayashidani, Yasuo

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Hiyama, Eiso

× Hiyama, Eiso

en Hiyama, Eiso

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Murakami, Yoshiaki

× Murakami, Yoshiaki

en Murakami, Yoshiaki

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Sueda, Taijiro

× Sueda, Taijiro

en Sueda, Taijiro

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
主題
主題Scheme Other
主題 Hammerhead ribozyme
主題
主題Scheme Other
主題 Telomerase
主題
主題Scheme Other
主題 Pancreas cancer
主題
主題Scheme NDC
主題 490
内容記述
内容記述 In cancer gene therapies, it is ideal to target tumor-specific genes. Since telomerase is activated in almost all cancer cells but not most somatic cells, it is considered as one of the favorite targets for cancer gene therapies. Ribozymes are catalytic RNA molecules with site-specific endoribonuclease activity. In the present study, we designed hammerhead ribozymes against human telomerase RNA (hTR) and human reverse transcriptase subunit (hTERT) to evaluate their effect on the attenuation of telomerase in the pancreas cancer cell line, PK-8. Hammerhead ribozyme targeting hTR depressed the level of telomerase activity in PK-8 cells. pRc-hTR vector with ribozyme targeting hTR and pRc-hTERT vector with ribozyme targeting hTERT mRNA were transfected into PK-8 cells and depressed telomerase activity and target RNA, but in pRc-hTR transfectant, hTERT mRNA expression was slightly upregulated and in pRc-hTERT transfectant hTR expression was also slightly upregulated. These findings indicate the co-regulation of hTR and hTERT mRNA expression in cancer cells. Extrachromosomal replicational vector, pCEP4, containing ribozyme targeting hTERT mRNA showed the most effective inhibition of telomerase activity, suggesting that the continuous effect of ribozyme is necessary to inhibit telomerase activity. Since the level of hTERT mRNA expression is less than that of hTR expression in cancer cells, ribozyme targeting hTERT mRNA might be a more useful therapeutic strategy for cancer gene therapy. Moreover, the co-regulation of hTR and hTERT mRNA expression in cancer cells to maintain the levels of telomerase activity suggested that the strategy of inhibiting hTERT mRNA and hTR simultaneously has a powerful potential as a gene therapy for targeting human telomerase.
言語 en
出版者
出版者 Hiroshima University Medical Press
出版者
出版者 国立情報学研究所
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ departmental bulletin paper
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0018-2052
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AA00664312
開始ページ
開始ページ 21
書誌情報 Hiroshima Journal of Medical Sciences
Hiroshima Journal of Medical Sciences

巻 54, 号 1, p. 21-27, 発行日 2005-03
旧ID 17696
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