| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Differential Display of messenger RNA Expressed Bronchoalveolar Lavage Cells in Pulmonary Sarcoidosis Patients |
|
言語 |
en |
| 作成者 |
Wiwien, Heru Wiyono
Hiyama, Keiko
Maeda, Hiroyuki
Ishioka, Shinichi
Yamakido, Michio
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Sarcoidosis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Bronchoalveolar lavage (BAL) cells |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Differential display |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
mRNA |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
Sarcoidosis is a systemic granulomatous disease of unknown origin. To clarify its pathogenesis, we searched for known or unknown genes which are specifically expressed in sarcoidosis. Bronchoalveolar lavage (BAL) cells from 18 patients with sarcoidosis and 8 patients with various lung diseases were analyzed by differential display method. mRNA was extracted from BAL cells and reverse transcribed with 12 kinds of anchored primer, which theoretically cover all mRNAs, followed by polymerase chain reaction (PCR) with the anchored primer and a 10-mer arbitrary primer. PCR products were displayed on a polyacrylamide gel and fragments showing characteristic alterations in intensity between sarcoidosis and other patients were extracted, sequenced, and compared against Genbank and EMEL DNA data bases. One fragment was detected with specifically increased intensity and another disappeared in patients with sarcoidosis. These fragments were likely derived from unknown genes. CD44 and tumor necrosis factor (TNF-α) cDNA sequences were also detected as fragments commonly expressed in sarcoidosis. The cloned fragments with specifically increased or decreased intensity in sarcoidosis may provide important information on the pathogenesis of sarcoidosis, and the display pattern implies the potential usefulness of this method as a tool for diagnosis of the disease. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This study was supported in part by research grants from Japanese Ministry of Education, Science and Culture and Japanese Ministry of Health and Welfare. |
| 出版者 |
|
|
出版者 |
Hiroshima University Medical Press |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0018-2052 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00664312 |
| 開始ページ |
|
|
開始ページ |
1 |
| 書誌情報 |
Hiroshima Journal of Medical Sciences
Hiroshima Journal of Medical Sciences
巻 45,
号 1,
p. 1-10,
発行日 1996-03
|
| 旧ID |
37833 |
HiroshimaJMedSci_45_1.pdf (1.1 MB)
