Automated Microanalysis of Creatinine by Coupled Enzyme Reactions

Asano, Kenji; Iwasaki, Hiroya; Fujimura, Katsuyuki; Ikeda, Mitsunori; Sugimoto, Yoshimi; Matsubara, Akemi; Yano, Kazuko; Irisawa, Hiroko; Kono, Fujiko; Kanbe, Masayuki; Tsubokura, Tokuo; Inouye, Yoshio; Nakamura, Shoshiro

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アイテム

Automated Microanalysis of Creatinine by Coupled Enzyme Reactions

https://hiroshima.repo.nii.ac.jp/records/2013296

名前 / ファイル	ライセンス	アクション
HiroshimaJMedSci_41_1.pdf (414.4 KB)

Item type

デフォルトアイテムタイプ_（フル）(1)

公開日

2023-03-18

タイトル

Automated Microanalysis of Creatinine by Coupled Enzyme Reactions

言語

作成者

Asano, Kenji
Iwasaki, Hiroya
Fujimura, Katsuyuki
Ikeda, Mitsunori
Sugimoto, Yoshimi
Matsubara, Akemi
Yano, Kazuko
Irisawa, Hiroko
Kono, Fujiko
Kanbe, Masayuki
Tsubokura, Tokuo
Inouye, Yoshio
Nakamura, Shoshiro

アクセス権

open access

アクセス権URI

http://purl.org/coar/access_right/c_abf2

主題

主題Scheme

Other

主題

Creatinine

主題

主題Scheme

Other

主題

Creatinine amidohydrolase

主題

主題Scheme

Other

主題

Creatine amidinohydrolase

主題

主題Scheme

Other

主題

Sarcosine oxidase

主題

主題Scheme

NDC

主題

490

内容記述

Hydrogen peroxide was generated from creatinine by the sequential enzyme reactions of creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase. Hydrogen peroxide was, in turn, used stoichiometrically for the condensation of 4-aminoantipyrine and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine catalyzed by horse-radish peroxidase, resulting in the formation of quinone dye with maximum absorbance at 546 nm. The optimized assay conditions for the enzymatic determination of creatinine in a HITACHI 7250 autoanalyzer was established. This system, which requires less than 5 μl of sample, was found to be the most economical for laboratories equipped with autoanalyzers.

言語

出版者

Hiroshima University Medical Press

言語

eng

資源タイプ

資源タイプ識別子

http://purl.org/coar/resource_type/c_6501

資源タイプ

departmental bulletin paper

出版タイプ

VoR

出版タイプResource

http://purl.org/coar/version/c_970fb48d4fbd8a85

Versions

Ver.1

2025-02-23 06:29:16.098161

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インデックスツリー

アイテム

Automated Microanalysis of Creatinine by Coupled Enzyme Reactions

× Asano, Kenji

× Iwasaki, Hiroya

× Fujimura, Katsuyuki

× Ikeda, Mitsunori

× Sugimoto, Yoshimi

× Matsubara, Akemi

× Yano, Kazuko

× Irisawa, Hiroko

× Kono, Fujiko

× Kanbe, Masayuki

× Tsubokura, Tokuo

× Inouye, Yoshio

× Nakamura, Shoshiro

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