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人赤血球中のアミノ酸・ポリアミン測定 : 第1 基礎編
https://hiroshima.repo.nii.ac.jp/records/2012853
https://hiroshima.repo.nii.ac.jp/records/20128539e407f96-57bd-4b08-8e35-f5f25a868357
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
HiroshimaJMedSci_32_113.pdf (1.1 MB)
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| Item type | デフォルトアイテムタイプ_（フル）(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-03-18 | |||||||
| タイトル | ||||||||
| タイトル | Determination of Amino Acids and Polyamines in Human Erythrocytes : Part 1. Fundamental Studies | |||||||
| 言語 | en | |||||||
| タイトル | ||||||||
| タイトル | 人赤血球中のアミノ酸・ポリアミン測定 : 第1 基礎編 | |||||||
| 言語 | ja | |||||||
| 作成者 |
Tokunaga, Akira
× Tokunaga, Akira
|
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| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Amino acid | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Polyamine | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Erythrocyte | |||||||
| 主題 | ||||||||
| 主題Scheme | NDC | |||||||
| 主題 | 490 | |||||||
| 内容記述 | ||||||||
| 内容記述 | 1) A simple, rapid analytical method for amino acids and polyamines in erythrocytes within a blood volume of l ml with a quantitative limit of precision ranging from 1-10 p moles was established using high performance liquid chromatography (HPLC) 2) A capillary sampling system (C-S system) which consists of a propylene capillary tube for hematocrit measurement was adopted for treatment of the erythrocytes. After fillihg the tube with blood, it was treated with a variety of method. 3) The free amino acids in erythrocytes had such characteristics as (1) high levels of acidic amino acids, (2) low levels _of Val, Met, Ile, Phe, Trp and Arg and (3) branched chain amino acids (Val, Ile, Leu) had a tendency to readily pass through the erythrocyte membrane. 4) Washing of erythrocyte pellets with saline was not good for measurement of amino acids in erythrocytes, particularly the branched chain amino acids. The space rate of non-washed erythrocyte pellets was calculated as (free amino acid levels in blood pellets-space rate x free amino acid levels in plasma). 5) Polyamine level in erythrocytes changes in accordance with aging of erythrocytes, i.e. it begins decreasing from the time reticulocytes are released from the bone marrow and continues throughout the course of mature erythrocytes up to the time of destruction in the spleen. | |||||||
| 言語 | en | |||||||
| 出版者 | ||||||||
| 出版者 | Hiroshima University School of Medicine | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連情報 | ||||||||
| 識別子タイプ | PMID | |||||||
| 関連識別子 | 6863007 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0018-2052 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA00664312 | |||||||
| 開始ページ | ||||||||
| 開始ページ | 113 | |||||||
| 書誌情報 |
Hiroshima Journal of Medical Sciences Hiroshima Journal of Medical Sciences 巻 32, 号 1, p. 113-126, 発行日 1983-03 |
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| 旧ID | 38366 | |||||||
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Cite as
Tokunaga, Akira, n.d., 人赤血球中のアミノ酸・ポリアミン測定 : 第1 基礎編: Hiroshima University School of Medicine, 113– p.
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