| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Exposure to positively- and negativelycharged plasma cluster ions impairs IgE-binding capacity of indoor cat and fungal allergens |
|
言語 |
en |
| 作成者 |
Nishikawa, Kazuo
Fujimura, Takashi
Ota, Yasuhiro
Abe, Takuya
ElRamlawy, Kareem Gamal
Nakano, Miyako
Takado, Tomoaki
Uenishi, Akira
Kawazoe, Hidechika
Sekoguchi, Yoshinori
Tanaka, Akihiko
Ono, Kazuhisa
Kawamoto, Seiji
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
© 2016 Nishikawa et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Airborne allergen |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Asp f 1 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Asthma |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Cat allergen |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Environmental control |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Fel d 1 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Fungal allergen |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Immunoglobulin E |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Positively- and negatively-charged cluster ions |
| 内容記述 |
|
|
内容記述 |
Background: Environmental control to reduce the amount of allergens in a living place is thought to be important to avoid sensitization to airborne allergens. However, efficacy of environmental control on inactivation of airborne allergens is not fully investigated. We have previously reported that positively- and negatively-charged plasma cluster ions (PC-ions) reduce the IgE-binding capacity of crude allergens from Japanese cedar pollen as important seasonal airborne allergens. Cat (Felis domesticus) and fungus (Aspergillus fumigatus) are also important sources of common airborne allergens in living spaces throughout the year, and early sensitization with those allergens is considered to be a risk factor for future development of allergic rhinitis, pollinosis and asthma. The aim of this study is to examine whether the PC-ions reduce the IgE-binding capacity of a cat major allergen (Fel d 1) and fungal allergens in an experimental condition. Methods: Fel d 1, crude fungal extract, or a fungal major allergen Asp f 1, was treated with PC-ions for 6 h in an experimental cylindrical apparatus. Sham-treated allergens were prepared in the same experimental apparatus without generation of PC-ions. The degradation of the PC-ions-treated Fel d 1 was analyzed by SDS-PAGE, and the IgE-binding capacity of the PC-ions-treated allergens was analyzed by ELISA inhibition assay. Results: Exposure of Fel d 1, crude fungal extract and Asp f 1 to PC-ions significantly decreased protein content of Fel d 1 or Asp f 1, respectively. SDS-PAGE analysis suggested that the decreased Fel d 1 content upon exposure with PC-ions was attributable to protein degradation. ELISA inhibition indicated that the PC-ions treatment significantly impaired IgE-binding capacities of Fel d 1, crude fungal allergens, and Asp f 1 compared to sham treatment. Discussion: Our data suggest that treatment with PC-ions not only reduce indoor cat and fungal allergens, but also impair their allergenicity. Conclusion: These results suggest that environmental control with PC-ions is useful for inactivation of indoor cat and fungal allergens. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This study was financially supported in part by the SHARP Corporation. |
| 出版者 |
|
|
出版者 |
BMC |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1186/s40413-016-0118-z |
| 関連情報 |
|
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
27660668 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1186/s40413-016-0118-z |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1939-4551 |
| 開始ページ |
|
|
開始ページ |
27 |
| 書誌情報 |
World Allergy Organization Journal
World Allergy Organization Journal
巻 9,
号 1,
p. 27,
発行日 2016-09-06
|
| 旧ID |
48669 |
WorldAllergyOrganJ_9_27.pdf (422.1 KB)
