| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Development of high-rate anaerobic ammonium-oxidizing (ANAMMOX) biofilm reactors |
|
言語 |
en |
| 作成者 |
Tsushima, Ikuo
Ogasawara, Yuji
Kindaichi, Tomonori
Satoh, Hisashi
Okabe, Satoshi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2007 Elsevier Ltd |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Anammox biofilm reactor |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
biofilm structure and function |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
16S rRNA approach |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
microelectrodes |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
430 |
| 内容記述 |
|
|
内容記述 |
To promptly establish anammox (anaerobic ammonium oxidation) reactors, appropriate seeding sludge with high abundance and activity of anammox bacteria was selected by quantifying 16S rRNA gene copy numbers of anammox bacteria by real-time quantitative PCR (RTQ-PCR) and batch culture experiments. The selected sludge was then inoculated into up-flow fixed-bed biofilm column reactors with nonwoven fabric sheets as biomass carrier and the reactor performances were monitored over one year. The anammox reaction was observed within 50 days and a total nitrogen removal rate of 26.0 kg-N m-3 day-1 was obtained after 247 days. To our knowledge, such a high rate has never been reported before. Hydraulic retention time (HRT) and influent NH4+ to NO2- molar ratio could be important determinant factors for efficient nitrogen removal in this study. The higher nitrogen removal rate was obtained at the shorter HRT and higher influent NH4+/NO2- molar ratio. After anammox reactors were fully developed, the community structure, spatial organization and in situ activity of the anammox biofilms were analyzed by the combined use of a full-cycle of 16S rRNA approach and microelectrodes. In situ hybridization results revealed that the probe Amx820-hybridized anaerobic anammox bacteria were distributed throughout the biofilm (accounting for more than 70% of total bacteria). They were associated with Nitrosomonas-like aerobic ammonia-oxidizing bacteria (AAOB) in the surface biofilm. The anammox bacteria present in this study were distantly related to the Candidatus Brocadia anammoxidans with the sequence similarity of 95%. Microelectrode measurements showed that a high in situ anammox activity (i.e., simultaneous consumption of NH4+ and NO2-) of 4.45 g-N of (NH4+ + NO2-) m-2 day-1 was detected in the upper 800 μm of the biofilm, which was consistent with the spatial distribution of anammox bacteria. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Pergamon-Elsevier Science Ltd. |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.watres.2007.01.050 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.watres.2007.01.050 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0043-1354 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00886427 |
| 開始ページ |
|
|
開始ページ |
1623 |
| 書誌情報 |
Water Research
Water Research
巻 41,
号 8,
p. 1623-1634,
発行日 2007-04
|
| 旧ID |
20587 |
WR_41-8_1623.pdf (597.5 KB)
