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Gene Targeting and Subsequent Site-Specific Transgenesis at the beta-actin (ACTB) Locus in Common Marmoset Embryonic Stem Cells

https://hiroshima.repo.nii.ac.jp/records/2008835
https://hiroshima.repo.nii.ac.jp/records/2008835
df037ca0-8433-4e09-bf46-918f046cfccb
名前 / ファイル ライセンス アクション
StemCellsDevelopment_20_1587.pdf StemCellsDevelopment_20_1587.pdf (1.6 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Gene Targeting and Subsequent Site-Specific Transgenesis at the beta-actin (ACTB) Locus in Common Marmoset Embryonic Stem Cells
言語 en
作成者 Shiozawa, Seiji

× Shiozawa, Seiji

en Shiozawa, Seiji

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Kawai, Kenji

× Kawai, Kenji

en Kawai, Kenji

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Okada, Yohei

× Okada, Yohei

en Okada, Yohei

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Tomioka, Ikuo

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en Tomioka, Ikuo

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Maeda, Takuji

× Maeda, Takuji

en Maeda, Takuji

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Kanda, Akifumi

× Kanda, Akifumi

en Kanda, Akifumi

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Shinohara, Haruka

× Shinohara, Haruka

en Shinohara, Haruka

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Suemizu, Hiroshi

× Suemizu, Hiroshi

en Suemizu, Hiroshi

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Okano, James Hirotaka

× Okano, James Hirotaka

en Okano, James Hirotaka

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Sotomaru, Yusuke

× Sotomaru, Yusuke

en Sotomaru, Yusuke

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Sasaki, Erika

× Sasaki, Erika

en Sasaki, Erika

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Okano, Hideyuki

× Okano, Hideyuki

en Okano, Hideyuki

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 (c) 2011 by Mary Ann Liebert, Inc. All Rights are Reserved.
主題
主題Scheme NDC
主題 490
内容記述
内容記述 Nonhuman primate embryonic stem (ES) cells have vast promise for preclinical studies. Genetic modification in nonhuman primate ES cells is an essential technique for maximizing the potential of these cells. The common marmoset (Callithrix jacchus), a nonhuman primate, is expected to be a useful transgenic model for preclinical studies. However, genetic modification in common marmoset ES (cmES) cells has not yet been adequately developed. To establish efficient and stable genetic modifications in cmES cells, we inserted the enhanced green fluorescent protein (EGFP) gene with heterotypic lox sites into the beta-actin (ACTB) locus of the cmES cells using gene targeting. The resulting knock-in ES cells expressed EGFP ubiquitously under the control of the endogenous ACTB promoter. Using inserted heterotypic lox sites, we demonstrated Cre recombinase-mediated cassette exchange (RMCE) and successfully established a monomeric red fluorescent protein (mRFP) knock-in cmES cell line. Further, a herpes simplex virus-thymidine kinase (HSV-tk) knock-in cmES cell line was established using RMCE. The growth of tumor cells originating from the cell line was significantly suppressed by the administration of ganciclovir. Therefore, the HSV-tk/ganciclovir system is promising as a safeguard for stem cell therapy. The stable and ubiquitous expression of EGFP before RMCE enables cell fate to be tracked when the cells are transplanted into an animal. Moreover, the creation of a transgene acceptor locus for site-specific transgenesis will be a powerful tool, similar to the ROSA26 locus in mice.
言語 en
出版者
出版者 Mary Ann Liebert, Inc.
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
関連情報
識別子タイプ DOI
関連識別子 10.1089/scd.2010.0351
関連情報
識別子タイプ DOI
関連識別子 http://dx.doi.org/10.1089/scd.2010.0351
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1547-3287
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AA11911253
開始ページ
開始ページ 1587
書誌情報 Stem Cells and Development
Stem Cells and Development

巻 20, 号 9, p. 1587-1600, 発行日 2011
旧ID 34740
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