| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2024-12-27 |
| タイトル |
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|
タイトル |
Effect of shading on trichome formation and CAPRICE-like gene expression in tea (Camellia sinensis var. sinensis) leaves |
|
言語 |
en |
| 作成者 |
Wakamatsu, Juri
Yamamoto, Mina
Kikuta, Mayumi
Tanaka, Wakana
Tominaga, Rumi
|
| アクセス権 |
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|
アクセス権 |
embargoed access |
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アクセス権URI |
http://purl.org/coar/access_right/c_f1cf |
| 権利情報 |
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|
言語 |
en |
|
権利情報 |
© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is not the published version. Please cite only the published version. |
| 権利情報 |
|
|
言語 |
ja |
|
権利情報 |
この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Phenylpropanoid |
| 主題 |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Shading |
| 主題 |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Tea leaf |
| 主題 |
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Transcription factor |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Trichome formation |
| 内容記述 |
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|
内容記述 |
Tea (Camellia sinensis var. sinensis) is one of the most popular beverages worldwide and is highly regarded for its unique taste and health benefits. The traditional practice of shading tea plants before harvest is employed to ensure the production of high-quality Japanese green tea. However, the precise effects of shading on trichome formation, leaf tissue structure, and the expression of related genes remain largely unknown. In the present study, we conducted a comparative analysis to investigate the effect of shading on the aforementioned aspects of tea leaves. Our findings revealed a significant decrease in the number of trichomes in the youngest 1st stage leaves under shaded conditions compared with that under full-light conditions. Histological analyses revealed that epidermal cells in tea leaves under shaded conditions were thinner, further indicating the influence of light on leaf structures. To delve deeper into the regulatory mechanisms, the expression levels of six Camellia sinensis CAPRICE (CsCPC) genes encoding R3 MYB transcription factors, which have been implicated in trichome formation in tea plants, were examined using real-time reverse transcription PCR. Among the six genes, CsCPC-1, Camellia sinensis ENHANCER OF TRY AND CPC 1–2 (CsETC1–2), and CsETC3 exhibited increased expression under shaded conditions, indicating their potential involvement in the light-mediated regulation of tea leaf characteristics. The expression levels of the phenylpropanoid pathway structural genes Camellia sinensis cinnamic acid 4-hydroxylase (CsC4H), Camellia sinensis cinnamoyl CoA reductase (CsCCR), and Camellia sinensis flavanone 3′ hydroxylase (CsF3′H), were significantly reduced under shaded conditions, suggesting the regulation of phenylpropanoid content. Our study demonstrated that shading treatment induces significant changes in the number of trichomes in younger leaves, alters the width of epidermal cells, and affects the expression levels of CsCPCs and genes related to the phenylpropanoid pathway. These findings have the potential to enhance tea leaf quality, and offer pragmatic implications for tea farming. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.scienta.2024.113049 |
| 助成情報 |
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助成機関名 |
日本学術振興会 |
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言語 |
ja |
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研究課題番号URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-22K05936/ |
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研究課題番号 |
22K05936 |
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研究課題名 |
CPCファミリーの機能解析を切り口とした、栄養欠乏時の根毛形成促進機構の解明 |
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言語 |
ja |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science |
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言語 |
en |
|
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研究課題番号URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-23KJ1651/ |
|
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研究課題番号 |
23KJ1651 |
|
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研究課題名 |
CPCファミリーの機能解析を切り口とした、栄養欠乏時の根毛形成促進機構の解明 |
|
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言語 |
ja |
| 助成情報 |
|
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助成機関名 |
日本学術振興会 |
|
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言語 |
ja |
|
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研究課題名 |
茶の品質を左右するトライコーム形成及びポリフェノール合成制御機構の解明 |
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言語 |
ja |
| 助成情報 |
|
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助成機関名 |
Japan Society for the Promotion of Science |
|
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言語 |
en |
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研究課題名 |
茶の品質を左右するトライコーム形成及びポリフェノール合成制御機構の解明 |
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言語 |
ja |
| 開始ページ |
|
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開始ページ |
113049 |
| 書誌情報 |
en : Scientia Horticulturae
巻 330,
p. 113049,
発行日 2024-03-01
|
| 旧ID |
55913 |
| 備考 |
The full-text file will be made open to the public on 1 March 2026 in accordance with publisher's 'Terms and Conditions for Self-Archiving' |
SciHort_330_113049.pdf (6.7 MB)
