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Studies of the role of steroid hormone in the regulation of oocyte maturation in cattle.

https://hiroshima.repo.nii.ac.jp/records/2008750
https://hiroshima.repo.nii.ac.jp/records/2008750
564b2103-f186-40e5-8f31-33e2e874b4dd
名前 / ファイル ライセンス アクション
1477-7827-4-4.pdf 1477-7827-4-4.pdf (328.2 KB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Studies of the role of steroid hormone in the regulation of oocyte maturation in cattle.
言語 en
作成者 Wang, Hai.Feng

× Wang, Hai.Feng

en Wang, Hai.Feng

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Isobe, Naoki

× Isobe, Naoki

en Isobe, Naoki

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Kumamoto, Kanako

× Kumamoto, Kanako

en Kumamoto, Kanako

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Yamashiro, Hideaki

× Yamashiro, Hideaki

en Yamashiro, Hideaki

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Yamashita, Yasuhisa

× Yamashita, Yasuhisa

en Yamashita, Yasuhisa

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Terada, Takato

× Terada, Takato

en Terada, Takato

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 Copyright (c) 2006 by Authors
主題
主題Scheme NDC
主題 460
内容記述
内容記述 Background: The objective of this study was to investigate whether the steroid hormone(s) secretedfrom cumulus-oocyte complexes (COCs) is a prerequisite for bovine oocyte maturation and cumulusexpansion using aminoglutethimide (AGT), a P450 cholesterol side-chain cleavage inhibitor.Methods: In experiment 1, COCs were cultured in maturation medium with various concentrations ofAGT for 22 h to determine the effective concentration of AGT to inhibit steroid hormone secretion,meiotic maturation and cumulus expansion. In experiment 2, COCs were cultured in conditioned medium(CM) and TCM-199 medium with or without 10 mM AGT to check whether steroid hormones secretedfrom COCs were responsible for oocyte maturation and cumulus expansion. Experiments 3 and 4 werecarried out to determine whether exogenous progesterone or estradiol-17beta was able to overcome theinhibitory effects of AGT on oocytes maturation and cumulus expansion. COCs cultured in 10 mM AGT-containing medium supplemented with various concentrations of progesterone or estradiol-17beta for 22h were examined for oocyte maturation and cumulus expansion. Results: Experiment 1 showed that a concentration of 10 mM AGT in medium was sufficient to blocksteroid hormone secretion, oocyte maturation and cumulus expansion, and that these inhibitory effectswere fully reversible. In experiment 2, the addition of 10 mM AGT to CM did not significantly preventoocyte maturation and cumulus expansion, implying that CM contains the steroid hormone(s) secretedfrom COCs, which are closely associated with oocyte maturation and cumulus expansion. The results inexperiments 3 and 4 demonstrated that the addition of any concentration of progesterone or estradiol-17beta in the medium did not reduce the inhibitory effects of AGT on oocyte maturation and cumulusexpansion.Conclusion: Our results indicate that bovine oocytes surrounded by cumulus cells are prevented frommaturation and cumulus expansion through the inhibition of steroid secretion due to AGT, and that theseinhibitory effects of AGT on oocyte maturation and cumulus expansions can not be overcome by theaddition of either progesterone or estradiol-17beta in the medium. These observations suggest that somesteroid hormone(s) other than P4 and E2 secreted from bovine COCs is essential for their meioticmaturation and cumulus expansion.
言語 en
出版者
出版者 Bio Med Central
日付
日付 2006
日付タイプ Created
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
関連情報
識別子タイプ DOI
関連識別子 10.1186/1477-7827-4-4
関連情報
識別子タイプ PMID
関連識別子 16457731
関連情報
識別子タイプ DOI
関連識別子 http://dx.doi.org/10.1186/1477-7827-4-4
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1477-7827
開始ページ
開始ページ 4
書誌情報 Reproductive biology and endocrinology
Reproductive biology and endocrinology

巻 4, p. 4, 発行日 2006
旧ID 15349
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