| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Role for CD47-SIRPα signaling in xenograft rejection by macrophages |
|
言語 |
en |
| 作成者 |
Ide, Kentaro
Wang, Hui
Tahara, Hiroyuki
Liu, Jianxiang
Wang, Xiaoying
Asahara, Toshimasa
Sykes, Megan
Yang, Yong-Guang
Ohdan, Hideki
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2007 the National Academy of Sciences |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
xenotransplantation |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
phagocytosis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
reticuloendothelial system |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
We have previously proven that human macrophages can phagocytose porcine cells even in the absence of Ab or complement opsonization, indicating that macrophages present a pivotal immunological obstacle to xenotransplantation. A recent report indicates that the signal regulatory protein (SIRP)α is a critical immune inhibitory receptor on macrophages, and its interaction with CD47, a ligand for SIRPα, prevents autologous phagocytosis. Considering the limited compatibility (73%) in amino acid sequences between pig and human CD47, we hypothesized that the interspecies incompatibility of CD47 may contribute to the rejection of xenogeneic cells by macrophages. In the present study, we have demonstrated that porcine CD47 does not induce SIRPα tyrosine phosphorylation in human macrophage-like cell line, and soluble human CD47-Fc fusion protein inhibits the phagocytic activity of human macrophages toward porcine cells. In addition, we have verified that manipulation of porcine cells for expression of human CD47 radically reduces the susceptibility of the cells to phagocytosis by human macrophages. These results indicate that the interspecies incompatibility of CD47 significantly contributes to the rejection of xenogeneic cells by macrophages. Genetic induction of human CD47 on porcine cells could provide inhibitory signaling to SIRPα on human macrophages, providing a novel approach to preventing macrophage-mediated xenograft rejection. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
National Academy of Sciences |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1073/pnas.0609661104 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
17360380 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1073/pnas.0609661104 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0027-8424 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10808769 |
| 開始ページ |
|
|
開始ページ |
5062 |
| 書誌情報 |
Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America
巻 104,
号 12,
p. 5062-5066,
発行日 2007-03-20
|
| 旧ID |
20739 |
PNAS_104-12_5062.pdf (174.9 KB)
