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The RNAi-mediated silencing of xanthine dehydrogenase impairs growth and fertility and accelerates leaf senescence in transgenic arabidopsis plants

https://hiroshima.repo.nii.ac.jp/records/2008658
https://hiroshima.repo.nii.ac.jp/records/2008658
284c76e1-7303-4905-8f0d-de9248355a4a
名前 / ファイル ライセンス アクション
PCP_48_1484.pdf PCP_48_1484.pdf (3.5 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル The RNAi-mediated silencing of xanthine dehydrogenase impairs growth and fertility and accelerates leaf senescence in transgenic arabidopsis plants
言語 en
作成者 Nakagawa, Ayami

× Nakagawa, Ayami

en Nakagawa, Ayami

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Sakamoto, Saori

× Sakamoto, Saori

en Sakamoto, Saori

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Takahashi, Misa

× Takahashi, Misa

en Takahashi, Misa

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Morikawa, Hiromichi

× Morikawa, Hiromichi

en Morikawa, Hiromichi

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Sakamoto, Atsushi

× Sakamoto, Atsushi

en Sakamoto, Atsushi

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 Copyright(c) 2007 Oxford University Press
主題
主題Scheme Other
主題 Arabidopsis (Arabidopsis thaliana)
主題
主題Scheme Other
主題 plant growth and development
主題
主題Scheme Other
主題 purine metabolism
主題
主題Scheme Other
主題 senescence
主題
主題Scheme Other
主題 xanthine dehydrogenase
主題
主題Scheme NDC
主題 460
内容記述
内容記述 Xanthine dehydrogenase (XDH) is a ubiquitous enzyme involved in purine metabolism which catalyzes the oxidation of hypoxanthine and xanthine to uric acid. Although the essential role of XDH is well documented in the nitrogen-fixing nodules of leguminous plants, the physiological importance of this enzyme remains uncertain in non-leguminous species such as Arabidopsis. To evaluate the impact of an XDH deficiency on whole-plant physiology and development in Arabidopsis, RNA interference (RNAi) was used to generate transgenic lines of this species in which AtXDH1 and AtXDH2, the two paralogous genes for XDH in this plant, were silenced simultaneously. The nearly complete reduction in the total XDH protein levels caused by this gene silencing resulted in the dramatic overaccumulation of xanthine and a retarded growth phenotype in which fruit development and seed fertility were also affected. A less severe silencing of XDH did not cause these growth abnormalities. The impaired growth phenotype was mimicked by treating wild-type plants with the XDH inhibitor allopurinol, and was reversed in the RNAi transgenic lines by exogenous supplementation of uric acid. Inactivation of XDH is also associated with precocious senescence in mature leaves displaying accelerated chlorophyll breakdown and by the early induction of senescence-related genes and enzyme markers. In contrast, the XDH protein levels increase with the aging of the wild-type leaves, supporting the physiological relevance of the function of this enzyme in leaf senescence. Our current results thus indicate that XDH functions in various aspects of plant growth and development.
言語 en
出版者
出版者 Oxford University Press
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ AO
出版タイプResource http://purl.org/coar/version/c_b1a7d7d4d402bcce
関連情報
識別子タイプ DOI
関連識別子 10.1093/pcp/pcm119
関連情報
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 http://dx.doi.org/10.1093/pcp/pcm119
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0032-0781
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AA0077511X
開始ページ
開始ページ 1484
書誌情報 Plant and Cell Physiology
Plant and Cell Physiology

巻 48, 号 10, p. 1484-1495, 発行日 2007-10
旧ID 21456
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