ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

  1. 学術雑誌論文等

Phospholipase D Family Member 4, a Transmembrane Glycoprotein with No Phospholipase D Activity, Expression in Spleen and Early Postnatal Microglia

https://hiroshima.repo.nii.ac.jp/records/2007936
https://hiroshima.repo.nii.ac.jp/records/2007936
e07946e5-ce75-4d89-895d-155298663a6a
名前 / ファイル ライセンス アクション
PLoS-One_5_e13932.pdf PLoS-One_5_e13932.pdf (3.2 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Phospholipase D Family Member 4, a Transmembrane Glycoprotein with No Phospholipase D Activity, Expression in Spleen and Early Postnatal Microglia
言語 en
作成者 Yoshikawa, Fumio

× Yoshikawa, Fumio

en Yoshikawa, Fumio

Search repository
Banno, Yoshiko

× Banno, Yoshiko

en Banno, Yoshiko

Search repository
Otani, Yoshinori

× Otani, Yoshinori

en Otani, Yoshinori

Search repository
Yamaguchi, Yoshihide

× Yamaguchi, Yoshihide

en Yamaguchi, Yoshihide

Search repository
Nagakura-Takagi, Yuko

× Nagakura-Takagi, Yuko

en Nagakura-Takagi, Yuko

Search repository
Morita, Noriyuki

× Morita, Noriyuki

en Morita, Noriyuki

Search repository
Sato, Yumi

× Sato, Yumi

en Sato, Yumi

Search repository
Saruta, Chihiro

× Saruta, Chihiro

en Saruta, Chihiro

Search repository
Nishibe, Hirozumi

× Nishibe, Hirozumi

en Nishibe, Hirozumi

Search repository
Sadakata, Tetsushi

× Sadakata, Tetsushi

en Sadakata, Tetsushi

Search repository
Shinoda, Yo

× Shinoda, Yo

en Shinoda, Yo

Search repository
Hayashi, Kanehiro

× Hayashi, Kanehiro

en Hayashi, Kanehiro

Search repository
Mishima, Yuriko

× Mishima, Yuriko

en Mishima, Yuriko

Search repository
Baba, Hiroko

× Baba, Hiroko

en Baba, Hiroko

Search repository
Furuichi, Teiichi

× Furuichi, Teiichi

en Furuichi, Teiichi

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 Copyright (c) 2010 Yoshikawa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
主題
主題Scheme NDC
主題 490
内容記述
内容記述 Background: Phospholipase D (PLD) catalyzes conversion of phosphatidylcholine into choline and phosphatidic acid, leading to a variety of intracellular signal transduction events. Two classical PLDs, PLD1 and PLD2, contain phosphatidylinositide-binding PX and PH domains and two conserved His-x-Lys-(x)4-Asp (HKD) motifs, which are critical for PLD activity. PLD4 officially belongs to the PLD family, because it possesses two HKD motifs. However, it lacks PX and PH domains and has a putative transmembrane domain instead. Nevertheless, little is known regarding expression, structure, and function of PLD4. Methodology/Principal Findings: PLD4 was analyzed in terms of expression, structure, and function. Expression was analyzed in developing mouse brains and non-neuronal tissues using microarray, in situ hybridization, immunohistochemistry, and immunocytochemistry. Structure was evaluated using bioinformatics analysis of protein domains, biochemical analyses of transmembrane property, and enzymatic deglycosylation. PLD activity was examined by choline release and transphosphatidylation assays. Results demonstrated low to modest, but characteristic, PLD4 mRNA expression in a subset of cells preferentially localized around white matter regions, including the corpus callosum and cerebellar white matter, during the first postnatal week. These PLD4 mRNA-expressing cells were identified as Iba1-positive microglia. In non-neuronal tissues, PLD4 mRNA expression was widespread, but predominantly distributed in the spleen. Intense PLD4 expression was detected around the marginal zone of the splenic red pulp, and splenic PLD4 protein recovered from subcellular membrane fractions was highly N-glycosylated. PLD4 was heterologously expressed in cell lines and localized in the endoplasmic reticulum and Golgi apparatus. Moreover, heterologously expressed PLD4 proteins did not exhibit PLD enzymatic activity. Conclusions/Significance: Results showed that PLD4 is a non-PLD, HKD motif-carrying, transmembrane glycoprotein localized in the endoplasmic reticulum and Golgi apparatus. The spatiotemporally restricted expression patterns suggested that PLD4 might play a role in common function(s) among microglia during early postnatal brain development and splenic marginal zone cells.
言語 en
出版者
出版者 Public Library of Science
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
関連情報
識別子タイプ DOI
関連識別子 10.1371/journal.pone.0013932
関連情報
識別子タイプ DOI
関連識別子 http://dx.doi.org/10.1371/journal.pone.0013932
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1932-6203
開始ページ
開始ページ e13932-1
書誌情報 PLoS ONE
PLoS ONE

巻 5, 号 11, p. e13932-1-e13932-13, 発行日 2010-11
旧ID 30865
戻る
0
views
See details
Views

Versions

Ver.1 2025-02-21 04:07:45.739580
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR 2.0
  • OAI-PMH JPCOAR 1.0
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3