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A Subset of Osteoblasts Expressing High Endogenous Levels of PPARc Switches Fate to Adipocytes in the Rat Calvaria Cell Culture Model

https://hiroshima.repo.nii.ac.jp/records/2007932
https://hiroshima.repo.nii.ac.jp/records/2007932
28aeb60e-8550-4aff-bd48-70fad35206f5
名前 / ファイル ライセンス アクション
PLoSONE_5_e11782.pdf PLoSONE_5_e11782.pdf (2.6 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル A Subset of Osteoblasts Expressing High Endogenous Levels of PPARc Switches Fate to Adipocytes in the Rat Calvaria Cell Culture Model
言語 en
作成者 Yoshiko, Yuji

× Yoshiko, Yuji

en Yoshiko, Yuji

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Oizumi, Kiyoshi

× Oizumi, Kiyoshi

en Oizumi, Kiyoshi

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Hasegawa, Takuro

× Hasegawa, Takuro

en Hasegawa, Takuro

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Minamizaki, Tomoko

× Minamizaki, Tomoko

en Minamizaki, Tomoko

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Tanne, Kazuo

× Tanne, Kazuo

en Tanne, Kazuo

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Maeda, Norihiko

× Maeda, Norihiko

en Maeda, Norihiko

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Aubin, Jane E.

× Aubin, Jane E.

en Aubin, Jane E.

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 © 2010 Yoshiko et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
内容記述
内容記述 Background: Understanding fate choice and fate switching between the osteoblast lineage (ObL) and adipocyte lineage (AdL) is important to understand both the developmental inter-relationships between osteoblasts and adipocytes and the impact of changes in fate allocation between the two lineages in normal aging and certain diseases. The goal of this study was to determine when during lineage progression ObL cells are susceptible to an AdL fate switch by activation of endogenous peroxisome proliferator-activated receptor (PPAR)c. Methodology/Principal Findings: Multiple rat calvaria cells within the ObL developmental hierarchy were isolated by either fractionation on the basis of expression of alkaline phosphatase or retrospective identification of single cell-derived colonies, and treated with BRL-49653 (BRL), a synthetic ligand for PPARc. About 30% of the total single cell-derived colonies expressed adipogenic potential (defined cytochemically) when BRL was present. Profiling of ObL and AdL markers by qRTPCR on amplified cRNA from over 160 colonies revealed that BRL-dependent adipogenic potential correlated with endogenous PPARc mRNA levels. Unexpectedly, a significant subset of relatively mature ObL cells exhibited osteoadipogenic bipotentiality. Western blotting and immunocytochemistry confirmed that ObL cells co-expressed multiple mesenchymal lineage determinants (runt-related transcription factor 2 (Runx2), PPARc, Sox9 and MyoD which localized in the cytoplasm initially, and only Runx2 translocated to the nucleus during ObL progression. Notably, however, some cells exhibited both PPARc and Runx2 nuclear labeling with concomitant upregulation of expression of their target genes with BRL treatment. Conclusions/Significance: We conclude that not only immature but a subset of relatively mature ObL cells characterized by relatively high levels of endogenous PPARc expression can be switched to the AdL. The fact that some ObL cells maintain capacity for adipogenic fate selection even at relatively mature developmental stages implies an unexpected plasticity with important implications in normal and pathological bone development.
言語 en
内容記述
内容記述タイプ Other
内容記述 This work was supported by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture of Japan (13771074 to YY) and the Canadian Institutes of Health Research (FRN 83704 to JEA). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
出版者
出版者 Public Library of Science
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
関連情報
識別子タイプ DOI
関連識別子 10.1371/journal.pone.0011782
関連情報
識別子タイプ PMID
関連識別子 20668686
関連情報
識別子タイプ DOI
関連識別子 https://doi.org/10.1371/journal.pone.0011782
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1932-6203
開始ページ
開始ページ e11782
書誌情報 PLoS ONE
PLoS ONE

巻 5, 号 7, p. e11782, 発行日 2010-07-26
旧ID 48983
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